Tang Tengteng, Casagrande Travis, Mohammadpour Pardis, Landis William, Lievers Brent, Grandfield Kathryn
Department of Materials Science and Engineering, McMaster University, Hamilton, Canada.
Center for Applied Biomechanics, Department of Mechanical and Aerospace Engineering, School of Engineering and Applied Science, University of Virginia, Charlottesville, USA.
Sci Rep. 2024 Sep 16;14(1):21604. doi: 10.1038/s41598-024-72739-8.
Three-dimensional correlative multimodal and multiscale imaging is an emerging method for investigating the complex hierarchical structure of biological materials such as bone. This approach synthesizes images acquired across multiple length scales, for the same region of interest, to provide a comprehensive view of the material structure of a sample. Here, we develop a workflow for the structural analysis of human trabecular bone using a femtosecond laser to produce a precise grid to facilitate correlation between imaging modalities and identification of structures of interest, in this case, a single trabecula within a volume of trabecular bone. Through such image registration, high resolution X-ray microscopy imaging revealed fine architectural details, including the cement sheath and bone cell lacunae of the selected bone trabecula. The selected bone volume was exposed with a combination of manual polishing and site-specific femtosecond laser ablation and then examined with plasma focused ion beam-scanning electron microscopy. This reliable and versatile correlation approach has the potential to be applied to a variety of biological tissues and traditional engineered materials. The proposed workflow has the enhanced capability for generating highly resolved and broadly contextualized structural data for a better understanding of the architectural features of a material spanning its macroscopic to nanoscopic levels.
三维相关多模态和多尺度成像是一种用于研究诸如骨骼等生物材料复杂层次结构的新兴方法。这种方法将针对同一感兴趣区域在多个长度尺度上获取的图像进行合成，以提供样本材料结构的全面视图。在此，我们开发了一种用于人体小梁骨结构分析的工作流程，使用飞秒激光生成精确网格，以促进成像模态之间的相关性以及感兴趣结构（在这种情况下是小梁骨体积内的单个小梁）的识别。通过这种图像配准，高分辨率X射线显微镜成像揭示了精细的结构细节，包括所选骨小梁的黏合线鞘和骨细胞陷窝。通过手动抛光和特定部位的飞秒激光烧蚀相结合的方式暴露所选骨体积，然后用等离子体聚焦离子束扫描电子显微镜进行检查。这种可靠且通用的相关方法有潜力应用于各种生物组织和传统工程材料。所提出的工作流程具有更强的能力，可生成高分辨率且具有广泛背景信息的结构数据，以便更好地理解材料从宏观到纳米尺度的结构特征。
