Terada N, Kuroda H, Matsumoto K, Kitamura Y
Jpn J Cancer Res. 1985 Jul;76(7):590-5.
Tubular adenomas developed spontaneously in ovaries of (WB X C57BL/6)F1-Sl/Slt and -W/Wv mice after 150 days of age. The uteri of the W/Wv mice were hypoplastic before development of tubular adenomas, but the uteri of the Sl/Slt mice were not. Since oophorectomy significantly reduced the uterus weight in the Sl/Slt mice, we investigated the androgen- and estrogen-producing activity of Sl/Slt and W/Wv mice before development of tubular adenomas. Fragments of ovaries were cultured for 48 hr in serum-free medium containing either progesterone or 4-androstene-3,17-dione (androstenedione). The amount of androgens produced from progesterone by ovaries of Sl/Slt mice in the presence of human chorionic gonadotropin was comparable to that of W/Wv mice. In contrast, the amount of estrogens produced from androstenedione by ovaries of Sl/Slt mice in the presence of follicle stimulating hormone was much greater than that of W/Wv mice. Moreover, the amount of estrogens produced was much greater than the amount of androgens in the ovaries of Sl/Slt mice. Therefore, before development of tubular adenomas, estrogens rather than androgens seem to be a major uterotropic hormone produced by ovaries of Sl/Slt mice.
150日龄后，（WB×C57BL/6）F1-Sl/Slt和-W/Wv小鼠的卵巢中自发形成管状腺瘤。在管状腺瘤发生之前，W/Wv小鼠的子宫发育不全，但Sl/Slt小鼠的子宫没有。由于卵巢切除术显著降低了Sl/Slt小鼠的子宫重量，我们研究了Sl/Slt和W/Wv小鼠在管状腺瘤发生之前产生雄激素和雌激素的活性。将卵巢组织块在含有孕酮或4-雄烯-3,17-二酮（雄烯二酮）的无血清培养基中培养48小时。在人绒毛膜促性腺激素存在的情况下，Sl/Slt小鼠卵巢从孕酮产生的雄激素量与W/Wv小鼠相当。相反，在促卵泡激素存在的情况下，Sl/Slt小鼠卵巢从雄烯二酮产生的雌激素量比W/Wv小鼠多得多。此外，Sl/Slt小鼠卵巢中产生的雌激素量远大于雄激素量。因此，在管状腺瘤发生之前，雌激素而非雄激素似乎是Sl/Slt小鼠卵巢产生的主要促子宫生长激素。
