Terada N, Kitamura Y, Namiki M, Kuroda H, Ito M, Takatsuka D, Matsumoto K
Cancer Res. 1984 May;44(5):1827-30.
Bilateral tubular adenomas develop spontaneously in ovaries of WB X C57BL/6 F1-Sl/Slt mice after exponential loss of oocytes. We investigated the ability of this tumor to produce sex steroids. Incubation of tubular adenoma tissue with [3H] progesterone resulted in production of [3H]androstenedione and [3H]-testosterone. The amount of androgens produced by the tumor tissue was much greater than that produced by the normal ovarian tissue. The concentration of testosterone in the serum of the Sl/Slt mice bearing tubular adenomas, measured by radioimmunoassay, was about five times as high as the value in the serum of the congenic +/+ mice. Although incubation of the tumor tissue with [3H]androstenedione resulted in production of 3H-estrogens, the aromatase activity of the tumor tissue was about one-fifth of that of the normal ovarian tissue. Therefore, the major sex steroid secreted by tubular adenomas seemed to be testosterone. These endocrinological features of tubular adenomas were consistent with the following pathological features of the Sl/Slt mice with the tumors: (a) although the weight of uterus of the Sl/Slt mice was about one-half of the value observed in the +/+ mice, it decreased markedly after oophorectomy; and (b) submandibular glands of the Sl/Slt mice were significantly heavier than those of the +/+ mice.
在卵母细胞呈指数减少后，WB×C57BL/6 F1-Sl/Slt小鼠的卵巢中会自发形成双侧管状腺瘤。我们研究了这种肿瘤产生性类固醇的能力。用[3H]孕酮孵育管状腺瘤组织会产生[3H]雄烯二酮和[3H]睾酮。肿瘤组织产生的雄激素量远大于正常卵巢组织产生的量。通过放射免疫测定法测量，携带管状腺瘤的Sl/Slt小鼠血清中的睾酮浓度约为同基因 +/+ 小鼠血清中睾酮浓度的五倍。尽管用[3H]雄烯二酮孵育肿瘤组织会产生3H-雌激素，但肿瘤组织的芳香化酶活性约为正常卵巢组织的五分之一。因此，管状腺瘤分泌的主要性类固醇似乎是睾酮。管状腺瘤的这些内分泌特征与患有肿瘤的Sl/Slt小鼠的以下病理特征一致：(a) 尽管Sl/Slt小鼠的子宫重量约为 +/+ 小鼠的一半，但在卵巢切除术后子宫重量显著下降；(b) Sl/Slt小鼠的下颌下腺明显比 +/+ 小鼠的下颌下腺重。
