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基于液相色谱-高分辨质谱的植物化学成分分析及抗氧化活性评价

Phytochemical profiling and antioxidant activity assessment of liquid chromatography-high-resolution mass spectrometry.

机构信息

Türk Telekom Science High School, Siirt, Siirt, Turkey.

出版信息

PeerJ. 2024 Sep 13;12:e18046. doi: 10.7717/peerj.18046. eCollection 2024.

Abstract

BACKGROUND

Plant-derived drugs are often preferred over synthetic drugs because of their superior safety profiles. Phenolic compounds and flavonoids-major plant components-possess antioxidant properties. Limited research has been conducted on the bioactive compounds and biochemical properties of (Asparagaceae), an important pharmacological species endemic to Turkey. Therefore, the chemical composition and antioxidant properties of were investigated in this study.

METHODS

The chemical composition of was analyzed using liquid chromatography-high-resolution mass spectrometry, and radical scavenging and antioxidant activities were evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)) tests.

RESULTS

Thirty-eight compounds were identified, including trans-cinnamic acid, caffeic acid, vitexin, schaftoside, orientin, and narirutin. showed high antioxidant activity in antioxidant activity tests.

CONCLUSION

These findings provide novel insights into the potential utility of in the pharmaceutical, food, and cosmetics industries, highlighted by its significant antioxidant capacity.

摘要

背景

由于植物衍生药物具有更好的安全性,因此常优先选择植物衍生药物而非合成药物。酚类化合物和类黄酮——植物的主要成分——具有抗氧化特性。对(天门冬科)这一土耳其特有重要药用物种的生物活性化合物和生化特性的研究有限。因此,本研究对 的化学成分和抗氧化特性进行了研究。

方法

使用液相色谱-高分辨率质谱法分析 的化学成分,并使用 DPPH(2,2-二苯基-1-苦基肼)和 ABTS(2,2'-联氮双-3-乙基苯并噻唑啉-6-磺酸)试验评估自由基清除和抗氧化活性。

结果

鉴定出 38 种化合物,包括反式肉桂酸、咖啡酸、牡荆素、荭草苷、木樨草苷和新橙皮苷。在抗氧化活性试验中,表现出很强的抗氧化活性。

结论

这些发现为 在制药、食品和化妆品行业的潜在应用提供了新的见解,其显著的抗氧化能力得到了强调。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28e6/11404456/dcdf39a31f4a/peerj-12-18046-g001.jpg

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