Hamana K, Kamekura M, Onishi H, Akazawa T, Matsuzaki S
J Biochem. 1985 Jun;97(6):1653-8. doi: 10.1093/oxfordjournals.jbchem.a135223.
Qualitative and quantitative determinations of polyamines have been done in 4 photosynthetic eubacteria and 6 extreme-halophilic archaebacteria. For comparison, 5 moderate-halophilic eubacteria were also analyzed to determine their polyamine contents. Not only putrescine and spermidine but also homospermidine were found in the photosynthetic eubacteria, especially in the N2-fixing species, Rhodospirillum and Chromatium. Norspermidine, norspermine, and spermine were not detected in the phototrophic eubacteria. No appreciable amount of any polyamine was found in extreme-halophilic archaebacteria, Halobacterium and Halococcus, while moderate-halophilic eubacteria contained quite high concentrations of putrescine and spermidine and cadaverine. When arginine was incubated with cell lysates of these two archaebacteria, appreciable amounts of agmatine were produced; neither putrescine nor cadaverine was formed in the presence of ornithine or lysine. No detectable amount of spermidine was produced by the lysates on incubation with putrescine.
已对4种光合真细菌和6种极端嗜盐古细菌进行了多胺的定性和定量测定。作为对照，还分析了5种中度嗜盐真细菌以确定它们的多胺含量。在光合真细菌中，尤其是在固氮物种红螺菌属和着色菌属中，不仅发现了腐胺和亚精胺，还发现了高同型亚精胺。在光合真细菌中未检测到降亚精胺、降精胺和精胺。在极端嗜盐古细菌盐杆菌属和盐球菌属中未发现任何可观数量的多胺，而中度嗜盐真细菌含有相当高浓度的腐胺、亚精胺和尸胺。当精氨酸与这两种古细菌的细胞裂解物一起孵育时，会产生可观数量的胍丁胺；在鸟氨酸或赖氨酸存在的情况下，既不形成腐胺也不形成尸胺。裂解物与腐胺一起孵育时，未检测到可检测量的亚精胺。
