Trojánek A, Kopanica M
J Chromatogr. 1985 Jun 28;328:127-33. doi: 10.1016/s0021-9673(01)87384-2.
A polarographic thin-layer detector for high-performance liquid chromatography, was devised. The detector has a sandwich structure with a working surface area of the mercury electrode of 0.25 mm2. The detector was applied to the direct amperometric detection and for detection with reactivation of the electrode surface by voltage pulses. It is shown that the latter detection mode is suitable for the determination of compounds that form insoluble or complex mercury salts, e.g., thiourea. Thiourea and its derivatives can be separated and determined in a wide concentration range and the detection limit for thiourea corresponds to 2 ng injected. Thiourea can be determined directly in urine.
设计了一种用于高效液相色谱的极谱薄层检测器。该检测器具有夹心结构，汞电极的工作表面积为0.25平方毫米。该检测器用于直接安培检测以及通过电压脉冲使电极表面再活化进行检测。结果表明，后一种检测模式适用于测定形成不溶性或络合汞盐的化合物，例如硫脲。硫脲及其衍生物可在很宽的浓度范围内进行分离和测定，硫脲的检测限相当于进样2纳克。硫脲可直接在尿液中测定。
