Guo Xintong, Du Xinyi, Zhao Gaoye, Liu Chongshen, Gao Jing, Huang Zunzhi, Dong Wei
School of Stomatology, North China University of Science and Technology, Tangshan, Hebei, China.
Oral Dis. 2025 Mar;31(3):741-755. doi: 10.1111/odi.15135. Epub 2024 Sep 17.
The odd-skipped related transcription factor 1 (OSR1) gene exerts distinct regulatory effects on tumorigenesis and development in various cancer types. However, the precise role of OSR1 in oral squamous cell carcinoma (OSCC) remains to be elucidated.
GEPIA 2 and TCGA databases were utilized to analyze the OSR1 expression in head and neck squamous cell carcinoma (HNSC) patients and its impact on prognosis. Hematoxylin-eosin staining, immunohistochemistry, immunofluorescence, western blotting, and RT-qPCR were employed to detect the OSR1 expression in OSCC tissues and cells. Lentivirus transfection was utilized for overexpression and downexpression of OSR1 in OSCC. CCK8 cell proliferation assay, colony formation and cell scratch assay were conducted to investigate the effects of OSR1 on biological behavior of OSCC cells. Western blotting and RT-qPCR were applied to investigate the regulatory mechanism of OSR1 on AXIN2/β-catenin signaling pathway.
OSR1 expression was significantly decreased in HNSC patients, OSCC tissues and cells, leading to a decrease in 5-year survival rate. OSR1 overexpression inhibited the proliferation and migration of OSCC cells, and the AXIN2/β-catenin signaling pathway was inhibited. Silencing OSR1 had the opposite effect.
OSR1 functioned as a tumor suppressor gene in OSCC proliferation and migration by regulating the AXIN2/β-catenin signaling pathway.
奇跳相关转录因子1（OSR1）基因在多种癌症类型的肿瘤发生和发展中发挥着不同的调节作用。然而，OSR1在口腔鳞状细胞癌（OSCC）中的确切作用仍有待阐明。
利用GEPIA 2和TCGA数据库分析头颈部鳞状细胞癌（HNSC）患者中OSR1的表达及其对预后的影响。采用苏木精-伊红染色、免疫组织化学、免疫荧光、蛋白质免疫印迹法和逆转录定量聚合酶链反应检测OSCC组织和细胞中OSR1的表达。利用慢病毒转染在OSCC中过表达和下调OSR1。进行CCK8细胞增殖试验、集落形成试验和细胞划痕试验，以研究OSR1对OSCC细胞生物学行为的影响。采用蛋白质免疫印迹法和逆转录定量聚合酶链反应研究OSR1对AXIN2/β-连环蛋白信号通路的调控机制。
HNSC患者、OSCC组织和细胞中OSR1表达显著降低，导致5年生存率下降。OSR1过表达抑制了OSCC细胞的增殖和迁移，AXIN2/β-连环蛋白信号通路受到抑制。沉默OSR1则产生相反的效果。
OSR1通过调节AXIN2/β-连环蛋白信号通路在OSCC增殖和迁移中发挥肿瘤抑制基因的作用。
