University of Science and Technology of China, Hefei 230026, China.
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.
Nano Lett. 2024 Oct 2;24(39):12233-12238. doi: 10.1021/acs.nanolett.4c03394. Epub 2024 Sep 17.
MicroRNAs (MiRNAs) are valuable biomarkers for the diagnosis and prognosis of diseases. The development of reliable assays is an urgent pursuit. We herein fabricate a novel electrochemical sensing strategy based on the conformation transitions of DNA nanostructures and click chemistry. Duplex-specific nuclease (DSN)-catalyzed reaction is first used for the disintegration of the DNA triangular pyramid frustum (DNA TPF). A DNA triangle is formed, which in turn assists strain-promoted alkyne-azide cycloaddition (SPAAC) to localize single-stranded DNA probes (P1). After SPAAC ligation, multiple DNA hairpins are spontaneously folded, and the labeled electrochemical species are dragged near the electrode interface. By recording and analyzing the responses, a highly sensitive electrochemical biosensor is established, which exhibits high sensitivity and reproducibility. Clinical applications have been verified with good stability. This sensing strategy relies on the integration of DNA nanostructures and click chemistry, which may inspire further designs for the development of DNA nanotechnology and applications in clinical chemistry.
微小 RNA（miRNAs）是疾病诊断和预后的有价值的生物标志物。开发可靠的检测方法是当务之急。本研究基于 DNA 纳米结构的构象转变和点击化学，构建了一种新型电化学传感策略。双链特异性核酸酶（DSN）催化反应首先用于破坏 DNA 三角金字塔截顶体（DNA TPF）。形成一个 DNA 三角形，它反过来协助应变促进的叠氮-炔环加成（SPAAC）将单链 DNA 探针（P1）定位。SPAAC 连接后，多个 DNA 发夹自动折叠，标记的电化学物质被拖到电极界面附近。通过记录和分析响应，建立了一种高灵敏度的电化学生物传感器，具有高灵敏度和重现性。临床应用具有良好的稳定性。这种传感策略依赖于 DNA 纳米结构和点击化学的整合，可能会激发进一步设计用于开发 DNA 纳米技术和在临床化学中的应用。
