Ma Yuzhu, Wang Tingting, Qu Xiaolin, Yan Ruhong, Miao Peng
Department of Clinical Laboratory, Suzhou Hospital, Affiliated Hospital of Medical School, Nanjing University, Suzhou 215153, China.
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.
Anal Chem. 2024 Dec 31;96(52):20348-20353. doi: 10.1021/acs.analchem.4c04744. Epub 2024 Dec 19.
Highly sensitive and accurate detection of disease biomarkers is of great importance for diagnosis, staging, and treatment of certain diseases. Herein, we report a novel electrochemical method for the quantification of miRNA biomarkers with DNA tetrahedrons as the signal reporters. Upon the initiation of DNA hairpin opening by miRNA at the electrode interface, the hidden click reaction group is exposed for the bioconjugation with a tetrahedral DNA nanostructure, which carries multiple electrochemical species. Strand displacement polymerization and reductant-mediated amplification are integrated for improved analytical performance. The established method achieves accurate quantitative detection of miRNA in the range from 100 aM to 10 pM. More importantly, it exhibits exceptional selectivity and stability, making it a highly convenient approach to monitor miRNA biomarkers, meeting the requirements of point-of-care testing.
高灵敏度和准确地检测疾病生物标志物对于某些疾病的诊断、分期和治疗至关重要。在此，我们报告了一种以DNA四面体作为信号报告分子来定量检测miRNA生物标志物的新型电化学方法。在电极界面处，miRNA引发DNA发夹打开，隐藏的点击反应基团暴露出来，用于与携带多个电化学活性物质的四面体DNA纳米结构进行生物共轭。整合链置换聚合和还原剂介导的扩增以提高分析性能。所建立的方法实现了对100 aM至10 pM范围内的miRNA进行准确的定量检测。更重要的是，它表现出卓越的选择性和稳定性，使其成为监测miRNA生物标志物的一种非常便捷的方法，满足即时检测的要求。
