Dallinga-Thie G M, Groot P H, van Tol A
J Lipid Res. 1985 Jul;26(7):889-92.
Methods for the quantitative determination of rat apolipoproteins A-I, A-IV, and E by electroimmunoassay are described. Apolipoproteins present in diluted samples of biological fluids (approx. 2 ml) were concentrated by precipitation with deoxycholate and trichloroacetic acid. The protein pellets were solubilized in 0.1 ml of 0.5 M NaOH and these samples were delipidated with tetramethylurea and assayed. This protocol enables the measurement of apolipoprotein concentrations that are at least 10 times lower than normally detectable; 0.2 micrograms of apolipoprotein A-IV, 0.2 micrograms of apolipoprotein A-I, and 0.8 micrograms of apolipoprotein E can be easily detected in samples of 2 ml.
本文描述了用电免疫测定法定量测定大鼠载脂蛋白A-I、A-IV和E的方法。生物体液稀释样本（约2ml）中的载脂蛋白通过脱氧胆酸盐和三氯乙酸沉淀进行浓缩。蛋白质沉淀用0.1ml 0.5M氢氧化钠溶解，这些样本用四甲基脲脱脂后进行测定。该方案能够测量至少比正常可检测浓度低10倍的载脂蛋白浓度；在2ml样本中可以轻松检测到0.2微克载脂蛋白A-IV、0.2微克载脂蛋白A-I和0.8微克载脂蛋白E。
