Ma Jilong, Ye Yiqian, He Rushi, Xiong Yan, Xiao Ruqi, Wang Kang, Zhang Youfa, Wu Xuewen
Department of Chemical and Pharmaceutical Engineering, School of Chemical Engineering, Xiangtan University, Xiangtan 411105, China.
Department of Chemical and Pharmaceutical Engineering, School of Chemical Engineering, Xiangtan University, Xiangtan 411105, China.
Food Chem. 2025 Jan 15;463(Pt 2):141281. doi: 10.1016/j.foodchem.2024.141281. Epub 2024 Sep 13.
Deoxynojirimycin (DNJ) is an α-glucosidase inhibitor with high food values. However, the complex and costly enrichment processes have greatly prevented its application. Herein, this study aimed to propose a simple and efficient enrichment process for DNJ from Morus alba L. extracts using cation exchange resins. The LSI and D113 resins were chosen due to their excellent adsorption and desorption properties. The adsorption characteristics agreed with the pseudo-first-order kinetic model and the Langmuir isotherm model. This adsorption was chemisorption, spontaneous, endothermic and entropy-driven. Furthermore, the concentration and pH of the extracts, desorption solvent, breakthrough and elution curves, sample loading and elution rate were investigated to optimize the enrichment process by resin column chromatography. The results also showed that the purity of DNJ was improved to 44.00 % with a total recovery of 78.21 % using the LSI-D113 combination strategy. This research demonstrated the industrial feasibility of DNJ enrichment using cation exchange resins.
脱氧野尻霉素（DNJ）是一种具有高营养价值的α-葡萄糖苷酶抑制剂。然而，复杂且昂贵的富集过程极大地阻碍了其应用。在此，本研究旨在提出一种使用阳离子交换树脂从桑白皮提取物中简单高效富集DNJ的方法。选择LSI和D113树脂是因为它们具有优异的吸附和解吸性能。吸附特性符合准一级动力学模型和朗缪尔等温线模型。这种吸附是化学吸附、自发的、吸热的且由熵驱动。此外，研究了提取物的浓度和pH值、解吸溶剂、穿透曲线和洗脱曲线、样品负载量和洗脱速率，以通过树脂柱色谱法优化富集过程。结果还表明，使用LSI-D113组合策略，DNJ的纯度提高到了44.00%，总回收率为78.21%。本研究证明了使用阳离子交换树脂富集DNJ的工业可行性。
