Zhao Yilan, Jiang Xue, Huang Ke, Xiong Xiaoli, Yang Qing
College of Chemistry and Material Science, Sichuan Normal University, Chengdu, Sichuan 610068, China.
College of Chemistry and Material Science, Sichuan Normal University, Chengdu, Sichuan 610068, China.
Food Chem. 2025 Jan 15;463(Pt 2):141280. doi: 10.1016/j.foodchem.2024.141280. Epub 2024 Sep 12.
A switchable ratiometric fluorescent smartphone-assisted sensing platform based on nitrogen-doped carbon dots (N-CDs) and Rhodamine B was fabricated for the determination of the ascorbic acid (AA) content in fruits by quenching the fluorescence of N-CDs with Hg (turn-off) and recovering with AA (turn-on). The blue-emission N-CDs was synthesized by liquid dielectric barrier discharge microplasma with an average size of 3.65 nm and an absolute quantum yield of 18 % (excited at 345 nm). In addition, the fluorescence color was converted to RGB values, enabling visual and quantitative determination of AA. Under optimal parameters, the linear ranges for detecting AA were found to be 3-170 μM and 5-170 μM for fluorescence spectrometer and smartphone sensing platform. The detection limits were 0.98 μM and 2.90 μM, respectively. Furthermore, the satisfactory recoveries in fruits were obtained by RF probe and smartphone platform. This smartphone-assisted platform will facilitate sensitive and visual determination for AA.
基于氮掺杂碳点（N-CDs）和罗丹明B构建了一种可切换的比率荧光智能手机辅助传感平台，用于通过汞猝灭N-CDs的荧光（关闭）和抗坏血酸（AA）恢复荧光（开启）来测定水果中的AA含量。通过液体介质阻挡放电微等离子体合成了蓝色发射的N-CDs，其平均尺寸为3.65 nm，绝对量子产率为18%（在345 nm激发）。此外，荧光颜色被转换为RGB值，实现了对AA的可视化和定量测定。在最佳参数下，荧光光谱仪和智能手机传感平台检测AA的线性范围分别为3-170 μM和5-170 μM。检测限分别为0.98 μM和2.90 μM。此外，通过射频探针和智能手机平台在水果中获得了令人满意的回收率。这种智能手机辅助平台将有助于对AA进行灵敏且可视化的测定。
