Islam M R, Manir M S, Razzak M, Mamun M A, Mortuza M F, Islam M J, Yang Shumin, Pan Huachun, Alam A K M M, Shubhra Quazi T H
Institute of Radiation and Polymer Technology, AERE, Bangladesh Atomic Energy Commission, Dhaka 1207, Bangladesh.
Materials Science Division, AECD, Bangladesh Atomic Energy Commission, Dhaka 1207, Bangladesh.
Int J Biol Macromol. 2024 Sep 15;280(Pt 1):135567. doi: 10.1016/j.ijbiomac.2024.135567.
This study explores the development of novel hydrogel composites for wound care, incorporating silk fibroin and reactive oxygen species (ROS)-scavenging dendrimers into a polyvinyl alcohol (PVA) matrix. Utilizing ionizing gamma radiation, we fabricated pristine PVA, silk-PVA (SPVA) binary, and dendrimer-silk-PVA (DSPVA) ternary hydrogel composites, with their composition confirmed via UV-visible absorption spectroscopy. Fourier-transform infrared (FTIR) and Raman spectroscopy analyses indicated complex interactions between the hydrogel components, enhancing their structural and biocompatible properties. Scanning electron microscopy (SEM) analysis revealed that dendrimer integration in DSPVA hydrogels significantly increased surface porosity, vital for tissue regeneration. The DSPVA hydrogels demonstrated effective ROS scavenging, reducing hydrogen peroxide (HO) concentrations by approximately 70 % within 24 h. In vivo wound healing studies in a diabetic mouse model showed enhanced wound closure in the DSPVA group, with a relative wound area reduction to 30 ± 4.3 % on day 10, compared to 56.5 ± 2.7 % in the control group. By the 16th day, the treated group exhibited near-complete wound contraction, markedly outperforming the control group. These findings underscore the potential of DSPVA hydrogels in diabetic wound management, combining silk fibroin's mechanical support, dendrimers' antioxidative properties, and PVA's structural benefits. Thus, DSPVA hydrogels are promising candidates for advanced wound care applications.
本研究探索用于伤口护理的新型水凝胶复合材料的开发，将丝素蛋白和活性氧（ROS）清除树枝状大分子纳入聚乙烯醇（PVA）基质中。利用电离伽马辐射，我们制备了原始PVA、丝素蛋白-PVA（SPVA）二元和树枝状大分子-丝素蛋白-PVA（DSPVA）三元水凝胶复合材料，并通过紫外可见吸收光谱法确认了它们的组成。傅里叶变换红外（FTIR）和拉曼光谱分析表明水凝胶成分之间存在复杂的相互作用，增强了它们的结构和生物相容性。扫描电子显微镜（SEM）分析显示，DSPVA水凝胶中树枝状大分子的整合显著增加了表面孔隙率，这对组织再生至关重要。DSPVA水凝胶表现出有效的ROS清除能力，在24小时内将过氧化氢（HO）浓度降低了约70%。在糖尿病小鼠模型中的体内伤口愈合研究表明，DSPVA组的伤口闭合得到增强，在第10天时相对伤口面积减少到30±4.3%，而对照组为56.5±2.7%。到第16天时，治疗组表现出近乎完全的伤口收缩，明显优于对照组。这些发现强调了DSPVA水凝胶在糖尿病伤口管理中的潜力，它结合了丝素蛋白的机械支撑、树枝状大分子的抗氧化特性和PVA的结构优势。因此，DSPVA水凝胶是先进伤口护理应用的有前途的候选材料。
