Lee Gyeongwoo, Ko Young-Gwang, Bae Ki Hyun, Kurisawa Motoichi, Kwon Oh Kyoung, Kwon Oh Hyeong
Department of Polymer Science and Engineering, Kumoh National Institute of Technology, Gumi, Gyeongbuk 39177, Korea.
Institute of Bioengineering and Bioimaging, 31 Biopolis Way, The Nanos, Singapore 138669, Singapore.
Biomater Res. 2022 Nov 9;26(1):62. doi: 10.1186/s40824-022-00304-3.
Overproduction of reactive oxygen species (ROS) is known to delay wound healing by causing oxidative tissue damage and inflammation. The green tea catechin, (-)-Epigallocatechin-3-O-gallate (EGCG), has drawn a great deal of interest due to its strong ROS scavenging and anti-inflammatory activities. In this study, we developed EGCG-grafted silk fibroin hydrogels as a potential wound dressing material.
The introduction of EGCG to water-soluble silk fibroin (SF-WS) was accomplished by the nucleophilic addition reaction between lysine residues in silk proteins and EGCG quinone at mild basic pH. The resulting SF-EGCG conjugate was co-crosslinked with tyramine-substituted SF (SF-T) via horseradish peroxidase (HRP)/HO mediated enzymatic reaction to form SF-T/SF-EGCG hydrogels with series of composition ratios.
Interestingly, SF-T70/SF-EGCG30 hydrogels exhibited rapid in situ gelation (< 30 s), similar storage modulus to human skin (≈ 1000 Pa) and superior wound healing performance over SF-T hydrogels and a commercial DuoDERM® gel dressings in a rat model of full thickness skin defect.
This study will provide useful insights into a rational design of ROS scavenging biomaterials for wound healing applications.
已知活性氧（ROS）的过度产生会通过引起氧化组织损伤和炎症来延迟伤口愈合。绿茶儿茶素（-）-表没食子儿茶素-3-没食子酸酯（EGCG）因其强大的ROS清除和抗炎活性而备受关注。在本研究中，我们开发了EGCG接枝的丝素蛋白水凝胶作为一种潜在的伤口敷料材料。
通过在温和碱性pH条件下丝蛋白中的赖氨酸残基与EGCG醌之间的亲核加成反应，将EGCG引入水溶性丝素蛋白（SF-WS）中。所得的SF-EGCG共轭物通过辣根过氧化物酶（HRP）/H₂O₂介导的酶促反应与酪胺取代的SF（SF-T）共交联，以形成具有一系列组成比例的SF-T/SF-EGCG水凝胶。
有趣的是，SF-T70/SF-EGCG30水凝胶表现出快速的原位凝胶化（<30秒），其储能模量与人皮肤相似（≈1000 Pa），并且在全层皮肤缺损大鼠模型中，其伤口愈合性能优于SF-T水凝胶和市售的DuoDERM®凝胶敷料。
本研究将为合理设计用于伤口愈合应用的ROS清除生物材料提供有用的见解。
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