Zhou Lixia, Sun Xiwei, Iqbal Amjad, Yarra Rajesh, Wu Qiufei, Li Jing, Lv Xiang, Ye Jianqiu, Yang Yaodong
Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences/Hainan Key Laboratory of Tropical Oil Crops Biology, Wenchang, Hainan, China; Hainan Coconut International Joint Research Center, Wenchang 571339, China.
Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences/Hainan Key Laboratory of Tropical Oil Crops Biology, Wenchang, Hainan, China; Department of Food Science & Technology, Abdul Wali Khan University Mardan, Pakistan; Hainan Coconut International Joint Research Center, Wenchang 571339, China.
Int J Biol Macromol. 2024 Sep 15;280(Pt 1):135699. doi: 10.1016/j.ijbiomac.2024.135699.
Aromatic coconut represents an exceptional variety of coconut known for its distinct and delightful flavor and aroma, both of which are highly cherished by consumers. Despite its popularity, there has been a lack of systematic research on aroma components and the associated synthetic genes. In this report, we developed the metabolite profiles of terpenoids by targeted metabolomics and obtained the expression profile of genes related to terpenoid biosynthesis by RNA-seq during different coconut fruit developmental stages. Totally, we separated 26 different terpenoids in aromatic coconut pulp, among which, geranyl acetate and (-)-isosyngene emerged as the most abundant. The integrated analysis of metabolism and RNA-seq data showed that HMGS2, HMGS3, IPI/IDI1, HMGR1, HMGR3, and CMK2 as potentially key genes involved in the synthesis of terpenoids in aromatic coconut. To validate these findings, qRT-PCR was conducted on terpenoid-related genes. These findings lay a foundation for understanding aroma formation and the molecular mechanism of terpenoids in coconut fruit.
香椰是一种特殊的椰子品种，以其独特而宜人的风味和香气而闻名，这两者都深受消费者喜爱。尽管它很受欢迎，但对其香气成分和相关合成基因缺乏系统的研究。在本报告中，我们通过靶向代谢组学开发了萜类化合物的代谢谱，并通过RNA测序获得了不同椰子果实发育阶段与萜类生物合成相关基因的表达谱。我们总共在香椰果肉中分离出26种不同的萜类化合物，其中乙酸香叶酯和（-）-异辛烯含量最为丰富。代谢组学和RNA测序数据的综合分析表明，HMGS2、HMGS3、IPI/IDI1、HMGR1、HMGR3和CMK2是香椰中萜类化合物合成的潜在关键基因。为了验证这些发现，我们对萜类相关基因进行了qRT-PCR。这些发现为理解椰子果实中香气形成和萜类化合物的分子机制奠定了基础。
