Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
JST, PRESTO, Saitama, Japan.
Adv Exp Med Biol. 2024;1461:97-108. doi: 10.1007/978-981-97-4584-5_7.
It has been known that cells have mechanisms to sense and respond to environmental noxiousness and mild temperature changes, such as heat shock response and thermosensitive TRP channels. Meanwhile, new methods of measuring temperature at the cellular level has recently been developed using fluorescent nanothermometers. Among these thermometers, fluorescent polymeric thermometers and fluorescent nanodiamonds excel in the properties required for intracellular thermometry. By using these novel methods to measure the temperature of single cells in cultures and tissues, it was revealed that spontaneous spatiotemporal temperature fluctuations occur within cells. Furthermore, the temperature fluctuations were related to organelles such as mitochondria and cellular and physiological functions, revealing a close relationship between intracellular temperature and cellular functions.
已知细胞具有感知和应对环境有害物质和温和温度变化的机制，例如热休克反应和热敏性 TRP 通道。同时，最近已经开发出使用荧光纳米温度计在细胞水平测量温度的新方法。在这些温度计中，荧光聚合物温度计和荧光纳米金刚石在细胞内测温所需的特性方面表现出色。通过使用这些新方法测量培养物和组织中单细胞的温度，发现在细胞内会发生自发的时空温度波动。此外，温度波动与细胞器（如线粒体）和细胞及生理功能有关，揭示了细胞内温度与细胞功能之间的密切关系。
