Uchiyama Seiichi, Gota Chie, Tsuji Toshikazu, Inada Noriko
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo 113-0033, Japan.
Chem Commun (Camb). 2017 Oct 5;53(80):10976-10992. doi: 10.1039/c7cc06203f.
In 2003, we successfully created the first fluorescent polymeric thermometer by combining a thermo-responsive polymer and an environment-sensitive (polarity and hydrogen bonding-sensitive) fluorophore. Its high sensitivity to temperature variation and high hydrophilicity, even under conditions of high ionic strength, enabled intracellular temperature measurements. Along with the progress of our research projects, the development of new luminescent molecular thermometers and the establishment of novel methods for measuring intracellular temperature have matured in this field. In this Feature Article, we summarize the background and history of intracellular temperature measurements using fluorescent polymeric thermometers based on studies performed in our laboratory and the relationship between our methods and those of other eminent research groups. Future research directions regarding intracellular temperature measurements are also discussed.
2003年，我们通过将热响应聚合物与环境敏感（对极性和氢键敏感）荧光团相结合，成功制造出首个荧光聚合物温度计。其对温度变化的高灵敏度以及即使在高离子强度条件下仍具有的高亲水性，使得细胞内温度测量成为可能。随着我们研究项目的推进，新型发光分子温度计的开发以及测量细胞内温度的新方法在该领域已日臻成熟。在这篇专题文章中，我们基于在我们实验室开展的研究，总结了使用荧光聚合物温度计进行细胞内温度测量的背景和历史，以及我们的方法与其他知名研究团队方法之间的关系。还讨论了细胞内温度测量未来的研究方向。
