The Key Oasis Eco-Agriculture Laboratory of Xinjiang Production and Construction Group, College of Agriculture, Shihezi University, Shihezi, Xinjiang, 832000, China.
CSIRO Agriculture and Food, Canberra, ACT, 2601, Australia.
BMC Plant Biol. 2024 Sep 18;24(1):870. doi: 10.1186/s12870-024-05571-x.
Secretory carrier membrane proteins (SCAMPs) form a family of integral membrane proteins and play a crucial role in mediating exocytosis in both animals and plants. While SCAMP genes have been studied in several plant species, their functions in cotton, particularly in response to abiotic stress, have not yet been reported.
In this study, a total of 53 SCAMP genes were identified in G. arboreum, G. raimondii, G. hirsutum, and G. barbadense. These genes were classified into five groups based on a phylogenetic analysis with SCAMPs from Arabidopsis thaliana. The main factor driving the expansion of the SCAMP gene family in G. hirsutum is tandem and segmental duplication events. Using MEME, in addition to the conserved SCAMP domain, we identified 3-13 other domains in each GhSCAMP. The cis-element analysis suggested that GhSCAMPs were widely involved in cotton growth and development, and responses to abiotic stresses. RNA sequencing (RNA-Seq) and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) results showed that most GhSCAMPs were expressed highly in many tissues and had differential expression responses to drought, cold, and heat stresses. Knock-down of GhSCAMP2 and GhSCAMP4 by virus-induced gene silencing (VIGS) lead to a salt-sensitive phenotype and had a lower content of CAT, POD, and SOD.
This study identified SCAMP genes in four cotton species, enhancing our understanding of the potential biological functions of SCAMPs. Additionally, we demonstrated that GhSCAMP2 and GhSCAMP4 positively regulate cotton tolerance to salt stress.
分泌载体膜蛋白（SCAMPs）构成了一个整合膜蛋白家族，在动物和植物中都发挥着介导胞吐作用的关键作用。虽然已经在几种植物物种中研究了 SCAMP 基因，但它们在棉花中的功能，特别是对非生物胁迫的响应，尚未有报道。
本研究在陆地棉、亚洲棉、雷蒙德氏棉和海岛棉中鉴定出了 53 个 SCAMP 基因。这些基因基于与拟南芥 SCAMPs 的系统发育分析被分为五个组。在陆地棉中，SCAMP 基因家族扩张的主要驱动因素是串联和片段重复事件。使用 MEME，除了保守的 SCAMP 结构域外，我们在每个 GhSCAMP 中鉴定出了 3-13 个其他结构域。顺式作用元件分析表明，GhSCAMPs 广泛参与棉花的生长发育以及对非生物胁迫的响应。RNA 测序（RNA-Seq）和反转录定量聚合酶链反应（RT-qPCR）结果表明，大多数 GhSCAMPs 在许多组织中高度表达，并且对干旱、寒冷和高温胁迫有不同的表达响应。通过病毒诱导的基因沉默（VIGS）敲低 GhSCAMP2 和 GhSCAMP4 导致盐敏感表型，并且 CAT、POD 和 SOD 的含量较低。
本研究在四个棉花物种中鉴定出了 SCAMP 基因，增强了我们对 SCAMPs 潜在生物学功能的理解。此外，我们证明了 GhSCAMP2 和 GhSCAMP4 正向调控棉花对盐胁迫的耐受性。
