Miao Qi, Zhang Min, He Aoyue, Qu Chuanyong, Zhang Rongqiang
Affiliated Hospital, Shaanxi University of Chinese Medicine, Shaanxi, Xianyang, 712000, PR China.
School of Public Health, Shaanxi University of Chinese Medicine, Shaanxi, Xianyang, 712046, PR China.
Heliyon. 2024 Sep 6;10(17):e37582. doi: 10.1016/j.heliyon.2024.e37582. eCollection 2024 Sep 15.
This study aimed to analyze the methylation pattern of deoxyribonucleic acid (CpG) sites in the _FA26 promoter region of patients with heart failure (HF) and explore the correlation between differential CpG methylation levels and various clinical parameters.
Peripheral blood specimens were collected from 20 patients with HF and 20 healthy individuals. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used to identify and detect the CpG sites in the _FA26 promoter region. CpG methylation levels were compared between patients with HF and healthy controls and patients with HF with different levels of cardiac function.
The methylation level of _FA26_CpG_17.18 in patients with HF was significantly lower than that in the healthy control group ( = 0.0002). Among patients with HF and cardiac function levels of I/II and III/IV, methylation levels of _FA26_CpG_24.25.26.27 ( = 0.0168) were significantly lower in those with III/IV cardiac function compared to those with I/II cardiac function.
The methylation level of _FA26_CpG_17.18 is significantly reduced in patients with HF, and that of _FA26_CpG_24.25.26.27 is significantly decreased in patients with III/IV cardiac function. Variations in _FA26 methylation levels influence coagulation, liver and kidney functions, and routine blood indexes, including D-dimer, albumin, calcium, and hemoglobin. This study provides clinical evidence for the involvement of _FA26 methylation in the occurrence and development of HF and proposes novel targets for HF prevention and treatment.
本研究旨在分析心力衰竭（HF）患者FA26启动子区域脱氧核糖核酸（CpG）位点的甲基化模式，并探讨差异CpG甲基化水平与各种临床参数之间的相关性。
收集20例HF患者和20例健康个体的外周血标本。采用基质辅助激光解吸/电离飞行时间质谱法鉴定和检测FA26启动子区域的CpG位点。比较HF患者与健康对照组以及不同心功能水平的HF患者之间的CpG甲基化水平。
HF患者中FA26_CpG_17.18的甲基化水平显著低于健康对照组（P = 0.0002）。在HF患者且心功能水平为I/II级和III/IV级的患者中，与心功能I/II级的患者相比，心功能III/IV级的患者中FA26_CpG_24.25.26.27的甲基化水平显著降低（P = 0.0168）。
HF患者中FA26_CpG_17.18的甲基化水平显著降低，心功能III/IV级的患者中FA26_CpG_24.25.26.27的甲基化水平显著降低。FA26甲基化水平的变化影响凝血、肝肾功能和血常规指标，包括D-二聚体、白蛋白、钙和血红蛋白。本研究为FA26甲基化参与HF的发生发展提供了临床证据，并为HF的预防和治疗提出了新的靶点。
