• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

河豚毒素及其相关分析方法的研究进展

Tetrodotoxin and the state-of-the-art progress of its associated analytical methods.

作者信息

Mi Wei, Liu Sha

机构信息

School of Public Health, Binzhou Medical University, Yantai, China.

出版信息

Front Microbiol. 2024 Sep 3;15:1413741. doi: 10.3389/fmicb.2024.1413741. eCollection 2024.

DOI:10.3389/fmicb.2024.1413741
PMID:39290516
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11407752/
Abstract

Tetrodotoxin (TTX), which is found in various marine organisms, including pufferfish, shellfish, shrimp, crab, marine gastropods, and gobies, is an effective marine toxin and the cause of many seafood poisoning incidents. Owing to its toxicity and threat to public health, the development of simple, rapid, and efficient analytical methods to detect TTX in various food matrices has garnered increasing interest worldwide. Herein, we reviewed the structure and properties, origin and sources, toxicity and poisoning, and relevant legislative measures of TTX. Additionally, we have mainly reviewed the state-of-the-art progress of analytical methods for TTX detection in the past five years, such as bioassays, immunoassays, instrumental analysis, and biosensors, and summarized their advantages and limitations. Furthermore, this review provides an in-depth discussion of the most advanced biosensors, including cell-based biosensors, immunosensors, and aptasensors. Overall, this study provides useful insights into the future development and wide application of biosensors for TTX detection.

摘要

河豚毒素（TTX）存在于各种海洋生物中，包括河豚、贝类、虾、蟹、海洋腹足类动物和虾虎鱼，是一种有效的海洋毒素，也是许多海鲜中毒事件的起因。由于其毒性以及对公众健康的威胁，开发简单、快速且高效的分析方法以检测各种食品基质中的河豚毒素已在全球范围内引起了越来越多的关注。在此，我们综述了河豚毒素的结构与性质、起源与来源、毒性与中毒情况以及相关立法措施。此外，我们主要回顾了过去五年中河豚毒素检测分析方法的最新进展，如生物测定法、免疫测定法、仪器分析法和生物传感器，并总结了它们的优缺点。此外，本综述深入讨论了最先进的生物传感器，包括基于细胞的生物传感器、免疫传感器和适配体传感器。总体而言，本研究为河豚毒素检测生物传感器的未来发展和广泛应用提供了有益的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/d3202e342336/fmicb-15-1413741-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/0dbdfab05964/fmicb-15-1413741-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/a133fe37660f/fmicb-15-1413741-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/d6cf97fdf801/fmicb-15-1413741-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/83a1f12c526b/fmicb-15-1413741-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/a6194ffff5e4/fmicb-15-1413741-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/1b91b047962e/fmicb-15-1413741-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/2154c72b6160/fmicb-15-1413741-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/229210a1b9bc/fmicb-15-1413741-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/4cb751bc1deb/fmicb-15-1413741-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/7d22f7ea9a6b/fmicb-15-1413741-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/1f59bf844dbd/fmicb-15-1413741-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/d3202e342336/fmicb-15-1413741-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/0dbdfab05964/fmicb-15-1413741-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/a133fe37660f/fmicb-15-1413741-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/d6cf97fdf801/fmicb-15-1413741-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/83a1f12c526b/fmicb-15-1413741-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/a6194ffff5e4/fmicb-15-1413741-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/1b91b047962e/fmicb-15-1413741-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/2154c72b6160/fmicb-15-1413741-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/229210a1b9bc/fmicb-15-1413741-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/4cb751bc1deb/fmicb-15-1413741-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/7d22f7ea9a6b/fmicb-15-1413741-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/1f59bf844dbd/fmicb-15-1413741-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f73/11407752/d3202e342336/fmicb-15-1413741-g012.jpg

相似文献

1
Tetrodotoxin and the state-of-the-art progress of its associated analytical methods.河豚毒素及其相关分析方法的研究进展
Front Microbiol. 2024 Sep 3;15:1413741. doi: 10.3389/fmicb.2024.1413741. eCollection 2024.
2
Tetrodotoxin and Its Analogues in Food: Recent Updates on Sample Preparation and Analytical Methods Since 2012.食品中的河豚毒素及其类似物：2012 年以来样品制备和分析方法的最新进展。
J Agric Food Chem. 2022 Oct 5;70(39):12249-12269. doi: 10.1021/acs.jafc.2c04106. Epub 2022 Sep 26.
3
Tetrodotoxin in marine bivalves and edible gastropods: A mini-review.海洋双壳贝类和食用腹足类中的河豚毒素：简要综述。
Chemosphere. 2019 Dec;236:124404. doi: 10.1016/j.chemosphere.2019.124404. Epub 2019 Jul 19.
4
Evaluation of surface plasmon resonance biosensors for detection of tetrodotoxin in food matrices and comparison to analytical methods.评价用于检测食品基质中河豚毒素的表面等离子体共振生物传感器，并与分析方法进行比较。
J Agric Food Chem. 2011 Feb 9;59(3):839-46. doi: 10.1021/jf103779k. Epub 2010 Dec 30.
5
The Incidence of Tetrodotoxin and Its Analogs in the Indian Ocean and the Red Sea.印度洋和红海地区的河豚毒素及其类似物的发生率。
Mar Drugs. 2019 Jan 5;17(1):28. doi: 10.3390/md17010028.
6
A fast magnetic bead-based colorimetric immunoassay for the detection of tetrodotoxins in shellfish.基于磁珠的快速比色免疫分析法检测贝类中的河豚毒素。
Food Chem Toxicol. 2020 Jun;140:111315. doi: 10.1016/j.fct.2020.111315. Epub 2020 Apr 5.
7
Tetrodotoxin poisoning due to pufferfish and gastropods, and their intoxication mechanism.河豚和腹足纲动物引起的河豚毒素中毒及其中毒机制。
ISRN Toxicol. 2011 Nov 30;2011:276939. doi: 10.5402/2011/276939. Print 2011.
8
Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples.自动膜片钳技术在检测河豚鱼样本中的河豚毒素中的应用。
Mar Drugs. 2024 Apr 15;22(4):176. doi: 10.3390/md22040176.
9
LC-MS/MS method for the determination of tetrodotoxin (TTX) on a triple quadruple mass spectrometer.用于在三重四极杆质谱仪上测定河豚毒素（TTX）的液相色谱-串联质谱法。
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2016 Nov;33(11):1728-1740. doi: 10.1080/19440049.2016.1235801. Epub 2016 Oct 17.
10
Tetrodotoxin--distribution and accumulation in aquatic organisms, and cases of human intoxication.河豚毒素——在水生生物中的分布与蓄积以及人类中毒案例
Mar Drugs. 2008 May 28;6(2):220-42. doi: 10.3390/md20080011.

引用本文的文献

1
An Update of Tetrodotoxins Toxicity and Risk Assessment Associated to Contaminated Seafood Consumption in Europe: A Systematic Review.欧洲因食用受污染海鲜导致的河豚毒素毒性及风险评估最新情况：一项系统综述
Toxins (Basel). 2025 Feb 8;17(2):76. doi: 10.3390/toxins17020076.
2
Tetrodotoxin: The State-of-the-Art Progress in Characterization, Detection, Biosynthesis, and Transport Enrichment.河豚毒素：表征、检测、生物合成及转运富集方面的最新进展
Mar Drugs. 2024 Nov 26;22(12):531. doi: 10.3390/md22120531.

本文引用的文献

1
A smartphone-based fluorescent biosensor with metal-organic framework biocomposites and cotton swabs for the rapid determination of tetrodotoxin in seafood.一种基于智能手机的荧光生物传感器，采用金属有机框架生物复合材料和棉签，用于快速测定海鲜中的河豚毒素。
Anal Chim Acta. 2024 Jul 4;1311:342738. doi: 10.1016/j.aca.2024.342738. Epub 2024 May 17.
2
Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples.自动膜片钳技术在检测河豚鱼样本中的河豚毒素中的应用。
Mar Drugs. 2024 Apr 15;22(4):176. doi: 10.3390/md22040176.
3
A label-free ratiometric fluorescent aptasensor based on a peroxidase-mimetic multifunctional ZrFe-MOF for the determination of tetrodotoxin.
基于过氧化物酶模拟多功能 ZrFe-MOF 的无标记比率荧光适体传感器用于河豚毒素的测定。
Mikrochim Acta. 2023 Dec 28;191(1):57. doi: 10.1007/s00604-023-06118-x.
4
Transcriptomic Profiling of Tetrodotoxin-Induced Neurotoxicity in Human Cerebral Organoids.人神经类器官中毒麻毒素诱导神经毒性的转录组学分析。
Mar Drugs. 2023 Nov 10;21(11):588. doi: 10.3390/md21110588.
5
Determination of tetrodotoxin in bivalve mollusks by hydrophilic interaction liquid chromatography-triple quadrupole mass spectrometry with internal standard calibration and its contamination in Zhejiang province, China.采用内标校准的亲水作用液相色谱-三重四极杆质谱法测定双壳贝类软体动物中的河豚毒素及其在中国浙江省的污染情况
Food Chem. 2024 Feb 15;434:137493. doi: 10.1016/j.foodchem.2023.137493. Epub 2023 Sep 16.
6
Laser induced graphene electrochemical aptasensor based on tetrahedral DNA for ultrasensitive on-site detection of microcystin-LR.基于四面体 DNA 的激光诱导石墨烯电化学适体传感器用于微囊藻毒素-LR 的超灵敏现场检测。
Biosens Bioelectron. 2023 Nov 1;239:115610. doi: 10.1016/j.bios.2023.115610. Epub 2023 Aug 18.
7
NIR-responsive photoelectrochemical sensing platform for the simultaneous determination of tetrodotoxin and okadaic acid in Nassariidae.用于同时测定海兔科中河豚毒素和 okadaic 酸的 NIR 响应光电流化学传感平台。
Food Chem. 2024 Jan 1;430:136999. doi: 10.1016/j.foodchem.2023.136999. Epub 2023 Jul 29.
8
Geographical differences in the composition of tetrodotoxin and 5,6,11-trideoxytetrodotoxin in Japanese pufferfishes and their origins.日本河豚鱼体内河豚毒素和 5,6,11-三去氧河豚毒素组成的地理差异及其来源。
Chemosphere. 2023 Sep;336:139214. doi: 10.1016/j.chemosphere.2023.139214. Epub 2023 Jun 14.
9
Sensitive and rapid detection of tetrodotoxin based on gold nanoflower-and latex microsphere-labeled monoclonal antibodies.基于金纳米花和乳胶微球标记单克隆抗体的河豚毒素灵敏快速检测
Front Bioeng Biotechnol. 2023 May 16;11:1196043. doi: 10.3389/fbioe.2023.1196043. eCollection 2023.
10
Determination of Multiple Neurotransmitters through LC-MS/MS to Confirm the Therapeutic Effects of Flower on TTX-Intoxicated Rats.通过 LC-MS/MS 测定多种神经递质，以确认花对 TTX 中毒大鼠的治疗效果。
Molecules. 2023 May 18;28(10):4158. doi: 10.3390/molecules28104158.