Mutuyemungu Erasme, Motta-Romero Hollman A, Yang Qinnan, Liu Sujun, Liu Sean, Singh Mukti, Rose Devin J
Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, NE, USA.
Nebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA.
Gut Microbiome (Camb). 2023 Dec 21;5:e1. doi: 10.1017/gmb.2023.18. eCollection 2024.
has been correlated with gas production by human faecal microbiota during fermentation. The objective of this study was to determine the role of in gas production by the microbiome. Kidney beans and sweet potatoes were subjected to digestion and dialysis followed by fermentation with ten faecal microbiomes: three with detectable (Me_D) and seven with no detectable (Me_ND). Me_D microbiomes produced more gas than the Me_ND microbiomes ( < 0.001). Me_D microbiomes produced more gas during fermentation of sweet potatoes than kidney beans ( < 0.001), while the opposite was true for the Me_ND microbiomes ( < 0.001). Among amplicon sequence variants that were associated with gas production, had the strongest association ( < 0.001). Me_D microbiomes consumed more acetate and produced more butyrate than Me_ND microbiomes ( < 0.001). Gas production by was confirmed by fermentation of sweet potatoes and acetate with human and rumen isolates. The human isolate produced gas on sweet potatoes and acetate. This study suggests that may be involved in gas production during the fermentation of flatulogenic foods through utilisation of undigestible substrates or cross-feeding on acetate.
已与人类粪便微生物群在发酵过程中的气体产生相关联。本研究的目的是确定[具体物质]在微生物群气体产生中的作用。对芸豆和红薯进行[具体消化方式]消化和透析，然后用十种粪便微生物群进行发酵：三种可检测到[具体物质]（Me_D），七种未检测到[具体物质]（Me_ND）。Me_D微生物群产生的气体比Me_ND微生物群多（P<0.001）。Me_D微生物群在红薯发酵过程中产生的气体比芸豆多（P<0.001），而Me_ND微生物群则相反（P<0.001）。在与气体产生相关的扩增子序列变体中，[具体物质]的关联最强（P<0.001）。Me_D微生物群比Me_ND微生物群消耗更多的乙酸盐并产生更多的丁酸盐（P<0.001）。通过用人和瘤胃[具体物质]分离物对红薯和乙酸盐进行发酵，证实了[具体物质]产生气体。人分离物在红薯和乙酸盐上产生气体。本研究表明，[具体物质]可能通过利用难消化的底物或对乙酸盐进行交叉喂养参与产气食物发酵过程中的气体产生。
