Zhai Xiaodong, Xue Yuhong, Song Wenjun, Sun Yue, Shen Tingting, Zhang Xinai, Li Yanxiao, Ding Fuyuan, Zhang Di, Zhou Chenguang, Arslan Muhammad, Tahir Haroon E, Li Zhihua, Shi Jiyong, Huang Xiaowei, Zou Xiaobo
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China.
Jiangsu Jicui Future Food Technology Research Institute, Yixing 214200, China.
Food Chem X. 2024 Sep 2;24:101801. doi: 10.1016/j.fochx.2024.101801. eCollection 2024 Dec 30.
Ratiometric fluorescent films with high amine sensitivity and stability were developed to monitor the freshness of beef and pork. Fluorescein isothiocyanate (FITC) and red carbon quantum dots (R-CQD) were used as the amine-responsive indicator and internal reference, respectively. The electrospun films prepared by immobilizing FITC and R-CQD complex (F-R) into polyvinylidene fluoride (PVDF) under 35 %, 55 % and 75 % of relative humidity (RH) were named F-R@PVDF-1, F-R@PVDF-2 and F-R@PVDF-3, respectively. In comparison, the F-R@PVDF-2 film exhibited the highest sensitivity to trimethylamine (TMA), demonstrating a limit of detection () value of 1.59 μM, and meanwhile high stability during storage with Δ value of 1.99 after 14 days of storage at 4 °C. The F-R@PVDF-2 film also showed a significant fluorescent red-to-brown color change during meat freshness monitoring at 4 °C. Conclusively, this study reported a new ratiometric fluorescent film that can be used to track the freshness of meats in food packaging.
开发了具有高胺敏感性和稳定性的比率荧光薄膜，用于监测牛肉和猪肉的新鲜度。分别使用异硫氰酸荧光素（FITC）和红色碳量子点（R-CQD）作为胺响应指示剂和内参。通过将FITC和R-CQD复合物（F-R）固定在聚偏氟乙烯（PVDF）中，在35%、55%和75%相对湿度（RH）下制备的电纺薄膜分别命名为F-R@PVDF-1、F-R@PVDF-2和F-R@PVDF-3。相比之下，F-R@PVDF-2薄膜对三甲胺（TMA）表现出最高的灵敏度，检测限（）值为1.59 μM，同时在4°C储存14天后具有高稳定性，Δ值为1.99。F-R@PVDF-2薄膜在4°C监测肉类新鲜度期间也显示出明显的荧光从红色到棕色的颜色变化。总之，本研究报道了一种新型比率荧光薄膜，可用于跟踪食品包装中肉类的新鲜度。
