Zhai Xiaodong, Ma Xingdan, Sun Yue, Xue Yuhong, Ban Wanwan, Song Wenjun, Shen Tingting, Li Zhihua, Huang Xiaowei, Sun Qing, Wu Kunlong, Chen Zhilong, Zou Wenwu, Liu Biao, Zhang Liang, Zhu Jiaji
Agricultural Product Processing and Storage Lab, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Wencheng Institution of Modern Agriculture and Healthcare Industry, Wencheng, Wenzhou 325300, China.
Foods. 2025 Jun 23;14(13):2200. doi: 10.3390/foods14132200.
A ratiometric fluorescent film with high gas sensitivity and stability was developed using electrospinning technology for monitoring food spoilage. 5(6)-Carboxyfluorescein (5(6)-FAM) was used as the indicator, combined with the internal reference Rhodamine B (RHB), to establish a composite ratiometric fluorescent probe (FAM@RHB). The hydrophobic fluorescent films were fabricated by incorporating FAM@RHB probes into polyvinylidene fluoride (PVDF) at varying molar ratios through electrospinning. The FR-2 film with a 2:8 ratio of 5(6)-FAM to RHB exhibited the best performance, demonstrating excellent hydrophobicity with a water contact angle () of 113.45° and good color stability, with a Δ value of 2.05 after 14 days of storage at 4 °C. Gas sensitivity tests indicated that FR-2 exhibited a limit of detection () of 0.54 μM for trimethylamine (TMA). In the application of monitoring the freshness of pork and beef at 4 °C, the fluorescence color of the FR-2 film significantly changed from orange-yellow to green, enabling the visual monitoring of meat freshness. Hence, this study provides a new approach for intelligent food packaging.
利用静电纺丝技术制备了一种具有高气体敏感性和稳定性的比率荧光薄膜，用于监测食品变质。以5(6)-羧基荧光素（5(6)-FAM）为指示剂，与内参罗丹明B（RHB）结合，建立了复合比率荧光探针（FAM@RHB）。通过静电纺丝将FAM@RHB探针以不同摩尔比掺入聚偏氟乙烯（PVDF）中，制备出疏水荧光薄膜。5(6)-FAM与RHB摩尔比为2:8的FR-2薄膜表现出最佳性能，具有113.45°的水接触角（），显示出优异的疏水性，在4℃储存14天后颜色稳定性良好，Δ值为2.05。气体敏感性测试表明，FR-2对三甲胺（TMA）的检测限（）为0.54μM。在4℃监测猪肉和牛肉新鲜度的应用中，FR-2薄膜的荧光颜色从橙黄色显著变为绿色，能够实现肉类新鲜度的可视化监测。因此，本研究为智能食品包装提供了一种新方法。
