Puspita Lesly, Juwono Virginia Blessy, Shim Jae-Won
Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Cheonan-si 31151, Korea.
Department of Integrated Biomedical Science, Soonchunhyang University, Cheonan-si 31151, Korea.
iScience. 2024 Aug 30;27(9):110856. doi: 10.1016/j.isci.2024.110856. eCollection 2024 Sep 20.
The capability of human pluripotent stem cells (hPSCs) to self-renew and differentiate into any cell type has greatly contributed to the advancement of biomedicine. Reporter lines derived from hPSCs have played a crucial role in elucidating the mechanisms underlying human development and diseases by acting as an alternative reporter system that cannot be used in living humans. To bring hPSCs closer to clinical application in transplantation, scientists have generated reporter lines for isolating the desired cell populations, as well as improving graft quality and treatment outcomes. This review presents an overview of the applications of hPSC reporter lines and the important variables in designing a reporter system, including options for gene delivery and editing tools, design of reporter constructs, and selection of reporter genes. It also provides insights into the prospects of hPSC reporter lines and the challenges that must be overcome to maximize the potential of hPSC reporter lines.
人类多能干细胞（hPSC）自我更新并分化为任何细胞类型的能力极大地推动了生物医学的发展。源自hPSC的报告基因系通过充当一种无法在活体人类中使用的替代报告系统，在阐明人类发育和疾病的潜在机制方面发挥了关键作用。为了使hPSC更接近移植的临床应用，科学家们已构建出报告基因系，用于分离所需的细胞群体，以及提高移植质量和治疗效果。本综述概述了hPSC报告基因系的应用以及设计报告系统中的重要变量，包括基因递送和编辑工具的选择、报告基因构建体的设计以及报告基因的选择。它还深入探讨了hPSC报告基因系的前景以及为最大限度发挥hPSC报告基因系的潜力而必须克服的挑战。
