Zhou Guangchen, Li Junda, Liang Xuhui, Yang Bohua, He Ximeng, Tang Hongyu, Guo Hongran, Liu Gongwei, Cui Wenyuan, Chen Yulin, Yang Yuxin
Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Anim Nutr. 2024 Apr 27;18:367-379. doi: 10.1016/j.aninu.2024.04.009. eCollection 2024 Sep.
This study was conducted to investigate potential regulatory mechanisms of feed efficiency (FE) in sheep by linking rumen microbiota with its host by the multi-omics analysis. One hundred and ninety-eight hybrid female sheep (initial body weight = 30.88 ± 4.57 kg; 4-month-old) were selected as candidate sheep. Each test sheep was fed in an individual pen for 60 days, and the residual feed intake (RFI) was calculated. The ten candidate sheep with the highest RFI were divided into the Low-FE group, and the ten with the lowest RFI were divided into the High-FE group, all selected for sample collection. The RFI, average daily gain and average daily feed intake were highly significantly different between the two experimental groups ( < 0.05). Compared with Low-FE group, the insulin-like growth factor-1 and very low-density lipoprotein in serum and the propionate in rumen significantly increased in High-FE group ( < 0.01), but the acetate:propionate ratio in rumen significantly decreased in High-FE group ( = 0.034). Metagenomics revealed sp. and were key bacteria, and increased abundance of the genes encoding the enzymes for cellulose degradation and production of propionate in High-FE group. The results of proteomics and section showed the rumen papilla length ( < 0.001) and expression of carbonic anhydrase and Na/K-ATPase were significantly higher in High-FE group ( < 0.05). On the other hand, the acetyl-CoA content significantly increased in the liver of High-FE group ( = 0.002). The relative expression levels of insulin-like growth factor-1 and apolipoprotein A4 genes were significantly up-regulated in the liver of High-FE group ( < 0.01), but relative expression level of monoacylglycerol O-acyltransferase 3 gene was significantly down-regulated ( = 0.037). These findings provide the mechanism by which the collaborative interaction between rumen microbiota fermentation and host uptake and metabolism of fermentation products impacts feed efficiency traits in sheep.
本研究旨在通过多组学分析将瘤胃微生物群与其宿主联系起来，探讨绵羊饲料效率（FE）的潜在调控机制。选择198只杂种雌性绵羊（初始体重=30.88±4.57千克；4月龄）作为候选绵羊。每只试验绵羊在个体栏中饲养60天，并计算剩余采食量（RFI）。将RFI最高的10只候选绵羊分为低饲料效率组，将RFI最低的10只绵羊分为高饲料效率组，所有这些绵羊均被选来进行样本采集。两个实验组之间的RFI、平均日增重和平均日采食量差异极显著（<0.05）。与低饲料效率组相比，高饲料效率组血清中的胰岛素样生长因子-1和极低密度脂蛋白以及瘤胃中的丙酸显著增加（<0.01），但高饲料效率组瘤胃中的乙酸:丙酸比值显著降低（=0.034）。宏基因组学显示，某菌属和某菌属是关键细菌，并且高饲料效率组中编码纤维素降解酶和丙酸生成酶的基因丰度增加。蛋白质组学和切片结果显示，高饲料效率组的瘤胃乳头长度（<0.001）以及碳酸酐酶和钠/钾-ATP酶的表达显著更高（<0.05）。另一方面，高饲料效率组肝脏中的乙酰辅酶A含量显著增加（=0.002）。高饲料效率组肝脏中胰岛素样生长因子-1和载脂蛋白A4基因的相对表达水平显著上调（<0.01），但单酰甘油O-酰基转移酶3基因的相对表达水平显著下调（=0.037）。这些发现揭示了瘤胃微生物群发酵与宿主对发酵产物的摄取和代谢之间的协同相互作用影响绵羊饲料效率性状的机制。
