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低EBI3表达促进胃癌的恶性程度。

Low EBI3 Expression Promotes the Malignant Degree of Gastric Cancer.

作者信息

Gu Qiqi, Wu Han, Cheng Zhouyang, Zhi Xiaofei, Song Qingjie, Chen Xiaoyang, Yang Xiaobing

机构信息

Department of General Surgery, The Affiliated Hospital of Nantong University, Jiangsu Province 226001, China.

Medical College of Nantong University, Nantong, Jiangsu Province 226001, China.

出版信息

Dis Markers. 2022 Mar 17;2022:5588043. doi: 10.1155/2022/5588043. eCollection 2022.

DOI:10.1155/2022/5588043
PMID:39291183
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11407894/
Abstract

OBJECTIVE

To explore expression changes and clinical significance of EBI3 in gastric cancer.

METHODS

Expression of EBI3 in gastric cancer (GC) cell lines, GC tissues, and corresponding adjacent tissues were detected by qRT-PCR, Western blot, or immunohistochemistry. The relationship between the EBI3 expression and clinicopathological features of GC patients was analyzed. Expression of EBI3 in BGC-823 was overexpressed or downregulated, then, the changes of proliferation, migration, invasion, and tumorigenicity of BGC-823 were observed by MTT, scratch test, Transwell test, and tumorigenesis assay model.

RESULTS

EBI3 was lowly expressed in GC tissues. EBI3 expression in BGC823 was highest than other cell lines. EBI3 expression was significantly associated with TNM stage. GC patients with low expression of EBI3 had a rather poor prognosis than the GC patients with high expression of EBI3. Low EBI3 expression was an independent risk predictor of the prognosis of GC patients. After EBI3 was overexpressed, the viability, migration, invasion, and tumorigenicity abilities of BGC-823 were significantly reduced. Opposite effect was observed after EBI3 expression was downregulated.

CONCLUSION

EBI3 low expression is closely related to the malignant degree of GC and may be a predictive indicator of the prognosis of GC and potential therapeutic targets.

摘要

目的

探讨EBI3在胃癌中的表达变化及其临床意义。

方法

采用qRT-PCR、蛋白质免疫印迹法或免疫组织化学法检测EBI3在胃癌（GC）细胞系、GC组织及相应癌旁组织中的表达。分析EBI3表达与GC患者临床病理特征之间的关系。对BGC-823细胞中EBI3进行过表达或下调表达，然后通过MTT法、划痕试验、Transwell试验及成瘤试验模型观察BGC-823细胞增殖、迁移、侵袭及成瘤能力的变化。

结果

EBI3在GC组织中低表达。EBI3在BGC823中的表达高于其他细胞系。EBI3表达与TNM分期显著相关。EBI3低表达的GC患者预后较EBI3高表达的GC患者差。EBI3低表达是GC患者预后的独立危险因素。EBI3过表达后，BGC-823细胞的活力、迁移、侵袭及成瘤能力显著降低。EBI3表达下调后则出现相反的作用。

结论

EBI3低表达与GC的恶性程度密切相关，可能是GC预后的预测指标及潜在的治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0430/11407894/502e241ea8f6/DM2022-5588043.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0430/11407894/8eeb0b0d814a/DM2022-5588043.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0430/11407894/6025e8db6fbf/DM2022-5588043.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0430/11407894/7ec2b7d9f1f4/DM2022-5588043.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0430/11407894/84e3073ef7ce/DM2022-5588043.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0430/11407894/502e241ea8f6/DM2022-5588043.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0430/11407894/8eeb0b0d814a/DM2022-5588043.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0430/11407894/6025e8db6fbf/DM2022-5588043.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0430/11407894/7ec2b7d9f1f4/DM2022-5588043.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0430/11407894/84e3073ef7ce/DM2022-5588043.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0430/11407894/502e241ea8f6/DM2022-5588043.005.jpg

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