Yuan Guanghui, Li Duan, Du Xin, Liu Yingxue, Wang Xiaoxiao, Hao Cuifang
Centre for Reproductive Medicine, Women and Children's Hospital, Qingdao University, Qingdao, China.
Branch of Shandong Provincial Clinical Research Center for Reproductive Health, Qingdao, China.
Front Cell Dev Biol. 2024 Sep 3;12:1445928. doi: 10.3389/fcell.2024.1445928. eCollection 2024.
Intrauterine transfusion of platelet-rich plasma (PRP) has become a new treatment for thin endometrium (TE) in recent years, but its low efficacy due to rapid release of growth factors limits its clinical use. Platelet-rich fibrin (PRF) starts the coagulation cascade reaction immediately after the blood comes into contact with the test tube. The natural coagulation process results in stable platelet activation and the slow release of growth factors.
In our study, primary human endometrial stromal cells (hESCs) were extracted from endometrial tissue. PRP and PRF were prepared from the patient cubital vein blood. Stromal cells were cultured in conditioned medium supplemented with PRP and PRF. Differences in cell behavior were observed by cell proliferation test and cell migration test. The relative expression levels of apoptotic Bax and antiapoptotic Bcl-2 genes were measured by qRT-PCR. The release of growth factors from PRP and PRF was detected by ELISA.
We found that both PRP and PRF inhibited apoptosis of hESCs, which favored cell proliferation and migration. In addition, PRF releases growth factors for a longer period of time compared to PRP.
PRF offer a more sustained therapeutic effect compared to PRP, which provides a new idea for endometrial regeneration and repair.
近年来，宫内输注富血小板血浆（PRP）已成为治疗薄型子宫内膜（TE）的一种新方法，但其生长因子快速释放导致疗效较低，限制了其临床应用。富血小板纤维蛋白（PRF）在血液与试管接触后立即启动凝血级联反应。自然凝血过程导致血小板稳定活化并缓慢释放生长因子。
在我们的研究中，从子宫内膜组织中提取原代人子宫内膜基质细胞（hESCs）。从患者肘静脉血制备PRP和PRF。将基质细胞在补充有PRP和PRF的条件培养基中培养。通过细胞增殖试验和细胞迁移试验观察细胞行为差异。通过qRT-PCR检测凋亡相关基因Bax和抗凋亡基因Bcl-2的相对表达水平。通过ELISA检测PRP和PRF中生长因子的释放。
我们发现PRP和PRF均抑制hESCs的凋亡，有利于细胞增殖和迁移。此外，与PRP相比，PRF释放生长因子的时间更长。
与PRP相比，PRF具有更持久的治疗效果，为子宫内膜再生和修复提供了新思路。
