一种能够阐明小分子磷脂膜黏附性和被动渗透参数的溶液 NMR 方法学。
A solution NMR methodology enabling the elucidation of small molecule phospholipid membrane adhesion and passive permeation parameters.
机构信息
School of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK.
School of Chemistry and Forensic Science, University of Kent, Canterbury, CT2 7NH, UK.
出版信息
Chem Commun (Camb). 2024 Oct 1;60(79):11160-11163. doi: 10.1039/d4cc01515k.
Quantifying small molecule uptake across a biological membrane of a target cell is crucial for the development of efficacious and selective drugs. However, current methods to obtaining such data are not trivial. Herein, we present an accessible, higher-throughput (20 minutes), H NMR spectroscopy assay, which enables the quantification of small molecule phospholipid passive membrane permeation and membrane adhesion parameters.
定量研究靶细胞生物膜对小分子的摄取对于开发有效和选择性药物至关重要。然而，目前获得此类数据的方法并不简单。在此，我们提出了一种易于使用的、高通量（20 分钟）的 1 H NMR 光谱测定法，可定量测定小分子磷脂的被动膜通透性和膜黏附参数。
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