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从应用角度选择合适的胶质母细胞瘤类器官生成方法的参考。

A reference for selecting an appropriate method for generating glioblastoma organoids from the application perspective.

作者信息

Liang Jing, He Peng

机构信息

Department of Operating Room, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, Sichuan, China.

Department of Biobank, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, Sichuan, China.

出版信息

Discov Oncol. 2024 Sep 18;15(1):459. doi: 10.1007/s12672-024-01346-w.

DOI:10.1007/s12672-024-01346-w
PMID:39292297
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11411047/
Abstract

Glioblastoma organoids (GBOs) serve as a powerful and reliable tool to study glioblastoma stem cells (GSCs) and glioblastoma (GBM). GBOs can be derived from different materials using different methods. To identify the predominant generation methods and the most applications of GBOs, we searched four databases (PubMed, Embase, Web of Science, and Wiley Online Laboratory) from August 2021 to August 2023. After screening, 42 out of 295 articles were included and analyzed. GBOs in these articles were generated using only one material, such as tumor tissues, tumor cells, and gene-edited multifunctional stem cells, or simultaneously using two materials, such as tumor cells and normal organoids. Methodologically, direct cultivation of GBM cells or tissues was the most commonly used method to generate GBOs. Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) were the frequently used multifunctional stem cells to generate GBOs by simultaneously silencing P53, NF1, and PTEN using CRISPR/Cas9. In terms of applications, GBOs generated by direct cultivation of GBM tissue had the most applications, including molecular mechanisms, therapy, and culture technique. This review provides a theoretical reference for selecting an appropriate method to generate GBOs when studying GSCs and GBM.

摘要

胶质母细胞瘤类器官（GBOs）是研究胶质母细胞瘤干细胞（GSCs）和胶质母细胞瘤（GBM）的强大而可靠的工具。GBOs可以使用不同方法从不同材料中获得。为了确定GBOs的主要生成方法及其最广泛的应用，我们在2021年8月至2023年8月期间检索了四个数据库（PubMed、Embase、Web of Science和Wiley Online Laboratory）。经过筛选，纳入并分析了295篇文章中的42篇。这些文章中的GBOs仅使用一种材料生成，如肿瘤组织、肿瘤细胞和基因编辑的多功能干细胞，或同时使用两种材料生成，如肿瘤细胞和正常类器官。在方法上,直接培养GBM细胞或组织是生成GBOs最常用的方法。胚胎干细胞（ESCs）和诱导多能干细胞（iPSCs）是常用的多功能干细胞，通过使用CRISPR/Cas9同时沉默P53、NF1和PTEN来生成GBOs。在应用方面，直接培养GBM组织生成的GBOs应用最为广泛，包括分子机制、治疗和培养技术。本综述为在研究GSCs和GBM时选择合适的方法生成GBOs提供了理论参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d27/11411047/71e3017f020a/12672_2024_1346_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d27/11411047/f7009438445f/12672_2024_1346_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d27/11411047/2f4dd922d01f/12672_2024_1346_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d27/11411047/00f8ef582b64/12672_2024_1346_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d27/11411047/71e3017f020a/12672_2024_1346_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d27/11411047/f7009438445f/12672_2024_1346_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d27/11411047/2f4dd922d01f/12672_2024_1346_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d27/11411047/00f8ef582b64/12672_2024_1346_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d27/11411047/71e3017f020a/12672_2024_1346_Fig4_HTML.jpg

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本文引用的文献

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The Aryl Hydrocarbon Receptor: Impact on the Tumor Immune Microenvironment and Modulation as a Potential Therapy.芳烃受体：对肿瘤免疫微环境的影响及作为潜在疗法的调节作用
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A multidimensional atlas of human glioblastoma-like organoids reveals highly coordinated molecular networks and effective drugs.
人类胶质母细胞瘤样类器官的多维图谱揭示了高度协调的分子网络和有效药物。
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