Shi Lei, Ruan Meng-Ling, Zhang Bing-Bing, Gong Guo-Xin, Li Xue-Wu, Refaie Alainaa, Sun Lv-Hui, Deng Zhang-Chao
State Key Laboratory of Agricultural Microbiology, Frontiers Science Center for Animal Breeding and Sustainable Production, Key Laboratory of Smart Farming Technology for Agricultural Animals of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Zhongke Jichuang New Material Technology Co., Ltd., Chengdu, 610000, China.
Biol Trace Elem Res. 2024 Sep 18. doi: 10.1007/s12011-024-04371-6.
This study aims to investigate the effect of different levels of zinc oxide quantum dots (ZnO-QDs) on the growth performance and gut health in broilers. A total of 1125 1-day-old Ross 308 broilers were randomly divided into five groups with 15 replicates of 15 chicks each. The broilers were fed basal diets supplemented with 0, 40, 80, 120, or 160 mg Zn/kg as ZnO-QDs for 6 weeks. The results showed that dietary 80 and 120 mg Zn/kg ZnO-QD supplementation increased (P < 0.05) average daily gain (1.4-1.7%) and reduced feed conversion ratio (1.3%) compared to the basal diet group during various experimental periods. Meanwhile, 80 mg Zn/kg ZnO-QD supplementation increased (P < 0.05) trypsin activity (25.4%), villus height, and the ratio of villus height to crypt depth in the jejunum. Moreover, 80 mg Zn/kg ZnO-QD supplementation increased (P < 0.05) the activities of glutathione reductase (47.7%) and superoxide dismutase (30.9%), while 120 mg Zn/kg ZnO-QD supplementation decreased (P < 0.05) glutathione peroxidase activity (27.1%) in the jejunum. Furthermore, 40 mg Zn/kg ZnO-QD supplementation down-regulated (P < 0.05) the expression of genes; interleukin-2, transforming growth factor β (TGF-β), Cathelicidin-1, Cathelicidin-2, Cathelicidin-3, and Occludin, while 80-160 mg Zn/kg ZnO-QD supplementation up-regulated (P < 0.05) Claudin-2 expression in the jejunum. In conclusion, dietary ZnO-QD supplementation improved growth performance of broilers potentially by enhancing their intestinal health status. Based on nonlinear regression analysis, the appropriate level of ZnO-QD supplementation would be from 98.2 to 102.5 mg Zn/kg.
本研究旨在探讨不同水平的氧化锌量子点（ZnO-QDs）对肉鸡生长性能和肠道健康的影响。总共1125只1日龄的罗斯308肉鸡被随机分为五组，每组15个重复，每个重复15只雏鸡。给肉鸡饲喂添加了0、40、80、120或160毫克锌/千克ZnO-QDs的基础日粮，持续6周。结果表明，在各个试验阶段，与基础日粮组相比，日粮中添加80和120毫克锌/千克的ZnO-QDs可提高（P < 0.05）平均日增重（1.4 - 1.7%）并降低饲料转化率（1.3%）。同时，添加80毫克锌/千克的ZnO-QDs可提高（P < 0.05）空肠中胰蛋白酶活性（25.4%）、绒毛高度以及绒毛高度与隐窝深度的比值。此外，添加80毫克锌/千克的ZnO-QDs可提高（P < 0.05）空肠中谷胱甘肽还原酶活性（47.7%）和超氧化物歧化酶活性（30.9%），而添加120毫克锌/千克的ZnO-QDs可降低（P < 0.05）空肠中谷胱甘肽过氧化物酶活性（27.1%）。此外，添加40毫克锌/千克的ZnO-QDs可下调（P < 0.05）白细胞介素-2、转化生长因子β（TGF-β）、Cathelicidin-1、Cathelicidin-2、Cathelicidin-3和闭合蛋白等基因的表达，而添加80 - 160毫克锌/千克的ZnO-QDs可上调（P < 0.05）空肠中Claudin-2的表达。总之，日粮中添加ZnO-QDs可能通过改善肠道健康状况来提高肉鸡的生长性能。基于非线性回归分析，ZnO-QDs的适宜添加水平为98.
