Duchossois Family Institute (DFI), University of Chicago, 900 E. 57th St., Chicago, IL 60637, USA; Department of Microbiology, Biological Sciences Division, University of Chicago, 5841 South Maryland Ave., Chicago, IL 60637, USA.
Duchossois Family Institute (DFI), University of Chicago, 900 E. 57th St., Chicago, IL 60637, USA.
Cell Host Microbe. 2024 Oct 9;32(10):1853-1867.e5. doi: 10.1016/j.chom.2024.08.016. Epub 2024 Sep 17.
Species of the Bacteroidales order are among the most abundant and stable bacterial members of the human gut microbiome, with diverse impacts on human health. We cultured and sequenced the genomes of 408 Bacteroidales isolates from healthy human donors representing nine genera and 35 species and performed comparative genomic, gene-specific, metabolomic, and horizontal gene transfer analyses. Families, genera, and species could be grouped based on many distinctive features. We also observed extensive DNA transfer between diverse families, allowing for shared traits and strain evolution. Inter- and intra-species diversity is also apparent in the metabolomic profiling studies. This highly characterized and diverse Bacteroidales culture collection with strain-resolved genomic and metabolomic analyses represents a valuable resource to facilitate informed selection of strains for microbiome reconstitution.
拟杆菌门的物种是人类肠道微生物组中最丰富和最稳定的细菌成员之一，对人类健康有多种影响。我们培养并测序了来自 9 个属和 35 个种的 408 个健康人类供体的拟杆菌门分离株的基因组，并进行了比较基因组、基因特异性、代谢组学和水平基因转移分析。可以根据许多独特的特征对科、属和种进行分组。我们还观察到不同家族之间广泛的 DNA 转移，允许共享特征和菌株进化。在代谢组学研究中也可以观察到种内和种间的多样性。这个具有高度特征和多样化的拟杆菌门培养物集合，具有菌株分辨率的基因组和代谢组学分析，是促进微生物组重建中菌株选择的有价值资源。
