Department of Biology and Chemistry, Justus-Liebig-University Giessen (JLU) Institute of Food Chemistry and Food Biotechnology, Heinrich-Buff-Ring 17, Giessen 35392, Germany.
Department Microbial Drugs and German Center for Infection Research (DZIF), partner side Hannover - Braunschweig, Helmholtz Centre for Infection Research GmbH (HZI), Inhoffenstrasse 7, Braunschweig 38124, Germany.
ACS Chem Biol. 2024 Oct 18;19(10):2144-2151. doi: 10.1021/acschembio.4c00178. Epub 2024 Sep 18.
is a model mushroom belonging to the fungal phylum Basidiomycota. Among others, is known for its diverse terpenome, containing various volatile and nonvolatile terpenes and terpenoids. Here, we deepen the knowledge on their biosynthetic pathways by studying the terpene synthase Agr2 in detail. In contrast to previous studies, the heterologous production of Agr2 in the agaric host revealed the production of two terpenes, one of which was the already known sesquiterpene viridiflorene. The other one was a so far unknown diterpene that had to be isolated and purified by means of preparative RP-HPLC for structure elucidation. 1D- and 2D-NMR experiments revealed the compound as the novel diterpene cyclocybene, pointing to the bifunctionality of Agr2 to produce both a sesquiterpene and a diterpene.
是一种属于真菌门担子菌纲的模式蘑菇。除其他外，因其多样的萜类化合物而闻名，其中包含各种挥发性和非挥发性萜类化合物和萜类化合物。在这里，我们通过详细研究萜烯合酶 Agr2 来加深对其生物合成途径的了解。与以前的研究相比，在担子菌宿主 中异源生产 Agr2 揭示了两种萜烯的产生，其中一种是已经知道的倍半萜类化合物青蒿烯。另一种是一种迄今为止未知的二萜类化合物，必须通过制备性反相高效液相色谱法进行分离和纯化，以进行结构阐明。1D 和 2D-NMR 实验表明该化合物为新型二萜类化合物环环倍半萜烯，表明 Agr2 具有产生半萜和二萜的双重功能。
