中国水蝎 Ranatra chinensis（半翅目：Nepidae）的染色体水平基因组组装。

Chromosome-level genome assembly of Chinese water Scorpion Ranatra chinensis (Heteroptera: Nepidae).

机构信息

Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, 100193, China.

College of Plant Protection, Northeast Agricultural University, Harbin, 150030, China.

出版信息

Sci Data. 2024 Sep 18;11(1):1016. doi: 10.1038/s41597-024-03856-2.

DOI:10.1038/s41597-024-03856-2

PMID:39294192

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11410988/

Abstract

Heteroptera (the true bugs), one of the most diverse lineages of insects, diversified in feeding strategies and living habitats, and thus become an ideal lineage for studies on adaptive evolution. Chinese water scorpion Ranatra chinensis (Heteroptera: Nepidae) is a predaceous bug living in lentic water systems, representing an ideal model for studying habitat transition and adaptation to water environment. However, genetic studies on this water bug remain limited. Here, we obtained a chromosome-level genome of R. chinensis using PacBio HiFi long reads and Hi-C sequencing reads. The total assembly size of genome is 867.89 Mb, with a scaffold N50 length of 26.48 Mb and the GC content of 39.50%. All contigs were assembled into 23 pseudo-chromosomes (N = 19 A + X1X2X3X4), and we predicted 18,424 protein-coding genes in this genome. This study will provide valuable genomic resources for future studies on the biology, water adaptation, and genome evolution of water bugs.

摘要

半翅目（真蝽）是昆虫中最多样化的谱系之一，其在取食策略和生活习性上呈现多样化，因此成为研究适应性进化的理想谱系。中国田鳖（Ranatra chinensis）（半翅目：负子蝽科）是一种生活在静水系统中的捕食性蝽，是研究生境转变和适应水环境的理想模型。然而，针对这种水生蝽的遗传研究仍然有限。本研究使用 PacBio HiFi 长读长和 Hi-C 测序技术，获得了中国田鳖的染色体水平基因组。基因组的总组装大小为 867.89Mb，支架 N50 长度为 26.48Mb，GC 含量为 39.50%。所有的 contigs 组装成 23 条假染色体（N=19A+X1X2X3X4），我们在这个基因组中预测了 18424 个蛋白质编码基因。本研究将为未来研究水生蝽的生物学、水适应和基因组进化提供有价值的基因组资源。