Hanoi National University of Education, 136 Xuan Thuy Street, Hanoi, Vietnam.
BMC Pediatr. 2024 Sep 18;24(1):594. doi: 10.1186/s12887-024-05073-7.
The Arg64 allele of the rs4994 (Trp64Arg) variant in the β3-adrenergic receptor (ADRB3) gene is involved in the control of energy balance by altering lipolysis and thermogenesis in adipocytes, ultimately contributing to the development of obesity. The objective of our study was to investigate the association between the rs4994 variant of the ADRB3 gene and obesity in Hanoi preschool-age children, adjusting for their eating behaviors.
A cross-sectional study was performed involving 708 children with normal weight and 304 children with obesity aged 3-5 years from 36 kindergartens in Hanoi, Vietnam. Cheek mucosa cell samples were used for DNA extraction, and genotyping at the ADRB3-rs4994 locus was performed using the polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP). Eating behaviors were assessed using the Children's Eating Behaviour Questionnaire (CEBQ). Binary logistic regression analysis was employed to examine the association between the rs4994 variant and obesity, adjusting for confounding factors such as age, sex, residence, birth weight, and eating behaviors.
The frequency of the C allele in the group with obesity was 16.4%, which was higher than in the control group (11.7%, P = 0.003). Children with the CC genotype exhibited significantly greater weight and weight-for-age Z-score compared to those with the TT and TC genotypes (P = 0.004 and 0.03, respectively). Following univariate and multivariate analyses adjusted for age, sex, residence, birth weight, and eating behaviors, a significant association between the rs4994 variant and obesity was observed (P < 0.05).
This study indicated that the ADRB3-rs4994 variant can be considered as an independent risk factor for obesity in Vietnamese preschool children.
β3-肾上腺素能受体（ADRB3）基因 rs4994（色氨酸 64 精氨酸）变异的 Arg64 等位基因通过改变脂肪细胞中的脂肪分解和产热来控制能量平衡，最终导致肥胖的发生。本研究的目的是在调整儿童饮食行为的基础上，探讨 ADRB3 基因 rs4994 变异与越南学龄前儿童肥胖的相关性。
采用横断面研究方法，选取越南河内 36 所幼儿园 3-5 岁正常体重儿童 708 名和肥胖儿童 304 名为研究对象。采集颊黏膜细胞样本提取 DNA，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测 ADRB3-rs4994 基因座的基因型。采用儿童饮食行为问卷（CEBQ）评估儿童饮食行为。采用二元逻辑回归分析调整年龄、性别、居住地、出生体重和饮食行为等混杂因素后，分析 rs4994 变异与肥胖的相关性。
肥胖组儿童 C 等位基因频率为 16.4%，高于对照组（11.7%，P=0.003）。CC 基因型儿童的体重和体重-年龄 Z 评分显著高于 TT 和 TC 基因型儿童（P=0.004 和 0.03）。在调整年龄、性别、居住地、出生体重和饮食行为等因素后，rs4994 变异与肥胖之间存在显著相关性（P<0.05）。
本研究表明，ADRB3-rs4994 变异可作为越南学龄前儿童肥胖的独立危险因素。
