Asai Mitsuo, Isshiki Nobuyuki, Takesue Masafumi, Takei Kuniharu
Performance Chemicals Research, Kao Corporation, Wakayama, 640-8580, Japan.
Graduate School of Information Science and Technology, Hokkaido University, Sapporo, Hokkaido, 060-0814, Japan.
Small Methods. 2025 Jan;9(1):e2401010. doi: 10.1002/smtd.202401010. Epub 2024 Sep 19.
Enzyme-based amperometric biosensors have become popular for healthcare applications. However, they have been under constant pressure for technological innovation to improve their sensitivity and usability. An ideal biosensor has high sensitivity and calibration-free characteristics. This study aims to report enzyme-based glucose and lactate sensors that utilize a proposed "time-derivative of potential (dOCP/dt)" method, with a further aim being to prove theoretically and experimentally that dOCP/dt values are proportional to substrate concentration. High sensitivity is obtained regardless of the electrode size because the electrode potential is independent of the electrode area in the biosensor. Importantly, because the substrate diffusion determines the enzyme reaction rate on the sensors, the dOCP/dt biosensors can essentially eliminate external influences such as temperature and pH. The result is the successful realization of a biosensor that is calibration-free, making it a much more practical option.
基于酶的安培生物传感器在医疗保健应用中已变得很受欢迎。然而，它们一直面临着持续的技术创新压力，以提高其灵敏度和可用性。理想的生物传感器具有高灵敏度和免校准特性。本研究旨在报告基于酶的葡萄糖和乳酸传感器，该传感器利用一种提出的“电位时间导数（dOCP/dt）”方法，进一步目标是从理论和实验上证明dOCP/dt值与底物浓度成正比。由于电极电位与生物传感器中的电极面积无关，因此无论电极尺寸如何都能获得高灵敏度。重要的是，由于底物扩散决定了传感器上的酶反应速率，dOCP/dt生物传感器基本上可以消除温度和pH等外部影响。结果是成功实现了一种免校准的生物传感器，使其成为更实用的选择。
