Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
National Center of Technology Innovation for Synthetic Biology, Tianjin, China.
Biotechnol J. 2024 Sep;19(9):e2400360. doi: 10.1002/biot.202400360.
Global climate deterioration intensifies the demand for exploiting efficient CO utilization approaches. Converting CO to biorefinery feedstock affords an alternative strategy for third-generation biorefineries. However, upcycling CO into complex chiral carbohydrates remains a major challenge. Previous attempts at sugar synthesis from CO either produce mixtures with poor stereoselectivity or require ATP as a cofactor. Here, by redesigning glycolaldehyde synthase, the authors constructed a synthetic pathway for biorefinery feedstock D-xylulose from CO that does not require ATP as a cofactor. The artificial D-xylulose pathway only requires a three-step enzyme cascade reaction to achieve the stereoselective synthesis of D-xylulose at a concentration of 1.2 g L. Our research opens up an alternative route toward future production of chemicals and fuels from CO.
全球气候恶化加剧了人们对开发高效 CO 利用方法的需求。将 CO 转化为生物炼制原料为第三代生物炼制厂提供了一种替代策略。然而，将 CO 高值化为复杂的手性碳水化合物仍然是一个主要挑战。以前从 CO 合成糖的尝试要么产生立体选择性差的混合物，要么需要 ATP 作为辅助因子。在这里，作者通过重新设计甘油醛 3-磷酸合酶，构建了一条从 CO 生物炼制原料 D-木酮糖的合成途径，该途径不需要 ATP 作为辅助因子。人工 D-木酮糖途径仅需三步酶级联反应，即可在 1.2 g/L 的浓度下实现 D-木酮糖的立体选择性合成。我们的研究为未来从 CO 生产化学品和燃料开辟了一条替代途径。
