Sun Shuai, Zhao Yuanyuan, Hao Yuxuan, Xue Pan, Guo Xiaoyang, Zhang Wei, Zhang Chunxiang, Zhang Qiongdi, Zhou Wenhua, Ong Hock Chun, Yu Xuefeng, Li Zeren, Li Jia, Yao Jianquan
Center for Intense Laser Application Technology, College of Engineering Physics, Shenzhen Technology University, Shenzhen 518118, China.
Interface Materials Center, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Biomed Opt Express. 2024 Aug 13;15(9):5215-5226. doi: 10.1364/BOE.535051. eCollection 2024 Sep 1.
A rapid, sensitive and easy-to-implement approach is proposed for the detection of pathogenic nucleic acids based on phase-based plasmonic spectroscopy of metallic gratings. The plasmonic sensors were fabricated using interference lithography and functionalized with single-stranded DNA probes to specific target SARS-CoV-2. The biosensor achieved the detection of 40 fM viral nucleic acids within 5 min; furthermore, a detection capability of 1 aM (0.6 copies/µL) was acquired when combining with the recombinase polymerase amplification. Additionally, the multiplexed sensing system was demonstrated to simultaneously detect three genomic sequences on a single sensor chip, thereby enhancing diagnostic accuracy and enabling high-throughput detection.
本文提出了一种基于金属光栅的相位等离子体光谱法快速、灵敏且易于实施的致病核酸检测方法。利用干涉光刻技术制备等离子体传感器，并用针对特定目标新冠病毒（SARS-CoV-2）的单链DNA探针进行功能化修饰。该生物传感器在5分钟内实现了对40 fM病毒核酸的检测；此外，与重组酶聚合酶扩增技术相结合时，检测能力达到了1 aM（0.6拷贝/µL）。此外，该多重传感系统被证明能够在单个传感器芯片上同时检测三个基因组序列，从而提高诊断准确性并实现高通量检测。
