Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal, 576 104, India; Centre of Excellence for Biophotonics, Manipal Academy of Higher Education, Manipal, 576 104, India.
Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal, 576 104, India; Centre for Applied Nanosciences, Manipal Academy of Higher Education, Manipal, 576 104, India.
Biosens Bioelectron. 2021 Apr 15;178:113004. doi: 10.1016/j.bios.2021.113004. Epub 2021 Jan 16.
The outbreak of life-threatening pandemic like COVID-19 necessitated the development of novel, rapid and cost-effective techniques that facilitate detection of viruses like SARS-CoV-2. The presently popular approach of a collection of samples using the nasopharyngeal swab method and subsequent detection of RNA using the real-time polymerase chain reaction suffers from false-positive results and a longer diagnostic time scale. Alternatively, various optical techniques namely optical sensing, spectroscopy, and imaging shows a great promise in virus detection. Herein, a comprehensive review of the various photonics technologies employed for virus detection, particularly the SARS-CoV family, is discussed. The state-of-art research activities in utilizing the photonics tools such as near-infrared spectroscopy, Fourier transform infrared spectroscopy, Raman spectroscopy, fluorescence-based techniques, super-resolution microscopy, surface plasmon resonance-based detection, for virus detection accounted extensively with an emphasis on coronavirus detection. Further, an account of emerging photonics technologies of SARS-CoV-2 detection and future possibilities is also explained. The progress in the field of optical techniques for virus detection unambiguously show a great promise in the development of rapid photonics-based devices for COVID-19 detection.
COVID-19 等危及生命的大流行病的爆发，需要开发新型、快速且具有成本效益的技术，以帮助检测 SARS-CoV-2 等病毒。目前流行的方法是使用鼻咽拭子法收集样本，然后使用实时聚合酶链反应检测 RNA，但这种方法存在假阳性结果和较长的诊断时间。另一方面，各种光学技术，如光学传感、光谱学和成像，在病毒检测方面显示出巨大的潜力。本文全面综述了用于病毒检测的各种光子学技术，特别是 SARS-CoV 家族。详细介绍了利用近红外光谱、傅里叶变换红外光谱、拉曼光谱、荧光技术、超分辨率显微镜、表面等离子体共振检测等光子学工具进行病毒检测的最新研究活动，并特别强调了冠状病毒检测。此外，还解释了 SARS-CoV-2 检测新兴光子学技术和未来可能性。光学技术在病毒检测方面的进展，无疑为开发基于快速光学的 COVID-19 检测设备带来了巨大的希望。
