Koedkanmark Thirawat, Ratchamak Ruthaiporn, Authaida Supakorn, Boonkum Wuttigrai, Semaming Yoswaris, Chankitisakul Vibuntita
Department of Animal Science, Faculty of Agricultural, Khon Kaen University, Khon Kaen, Thailand.
Network Center for Animal Breeding and Omics Research, Khon Kaen University, Khon Kaen, Thailand.
Front Vet Sci. 2024 Sep 4;11:1474386. doi: 10.3389/fvets.2024.1474386. eCollection 2024.
Cooled semen storage methods result in oxidative stress generated by an imbalance between oxidation rates, specifically reactive oxygen species production, and sperm cell antioxidants, leading to degradation of semen quality. We aimed to investigate the impact of adding Eurycoma longifolia (EL) extract as an antioxidant supplement in semen storage medium (IGGKPh semen extender) on semen quality and fertility potential. EL extract at concentrations of 5, 10, 15, and 20 mg/mL was assessed for its antioxidant capacity in IGGKPh semen extender. Our findings revealed that the total phenolic content in the EL extract did not vary significantly across the various concentrations and temperatures tested. However, incubation at 5°C was found to be the most effective temperature for increasing the EL extract antioxidant capacity as assessed via the 2,2-diphenyl-1-picrylhydrazyl inhibition assay in a dose-dependent manner. Supplementation of the IGGKPh semen extender with 15 mg/mL EL extract was found to enhance semen quality during cold storage for up to 48 h ( < 0.05), as evidenced by decreased malondialdehyde levels in cooled semen ( < 0.05). However, antioxidant enzyme activities showed no significant differences among the various experimental groups ( > 0.05). The fertility test showed that the 15 mg/mL EL extract group stored for 24 h had a higher percentage than the control group ( < 0.05). However, there was no significant difference in percentage between the two groups at 48 h of storage ( > 0.05). The hatchability showed no significant difference in both 24 and 48-h storage periods ( > 0.05). Our results indicated that supplementing the IGGKPh semen extender with 15 mg/mL EL extract may positively influence semen quality during storage, suggesting potential applications for enhancing semen quality.
精液冷藏方法会导致氧化应激，这是由氧化速率（特别是活性氧的产生）与精子细胞抗氧化剂之间的失衡所引发的，进而导致精液质量下降。我们旨在研究在精液保存介质（IGGKPh精液稀释液）中添加长叶刺蒺藜提取物（EL）作为抗氧化剂补充剂对精液质量和生育潜力的影响。对浓度为5、10、15和20mg/mL的EL提取物在IGGKPh精液稀释液中的抗氧化能力进行了评估。我们的研究结果表明，在测试的不同浓度和温度下，EL提取物中的总酚含量没有显著差异。然而，通过2,2-二苯基-1-苦基肼抑制试验评估发现，在5°C下孵育是提高EL提取物抗氧化能力最有效的温度，且呈剂量依赖性。在IGGKPh精液稀释液中添加15mg/mL的EL提取物可在冷藏长达48小时期间提高精液质量（<0.05），冷藏精液中的丙二醛水平降低（<0.05）证明了这一点。然而，各实验组之间的抗氧化酶活性没有显著差异（>0.05）。生育力测试表明，储存24小时的15mg/mL EL提取物组的百分比高于对照组（<0.05）。然而，在储存48小时时，两组之间的百分比没有显著差异（>0.05）。孵化率在24小时和48小时的储存期内均无显著差异（>0.05）。我们的结果表明，在IGGKPh精液稀释液中添加15mg/mL的EL提取物可能会对储存期间的精液质量产生积极影响，这表明其在提高精液质量方面具有潜在应用价值。
