Koedkanmark Thirawat, Buathalad Khomsan, Authaida Supakorn, Boonkum Wuttigrai, Chankitisakul Vibuntita
Department of Animal Science, Faculty of Agricultural, Khon Kaen University, Khon Kaen 40002, Thailand.
Department of Animal Science, Faculty of Agricultural, Khon Kaen University, Khon Kaen 40002, Thailand; Network Center for Animal Breeding and Omics Research, Khon Kaen University, Khon Kaen 40002, Thailand.
Poult Sci. 2025 Apr;104(4):104902. doi: 10.1016/j.psj.2025.104902. Epub 2025 Feb 19.
Cryopreservation of rooster semen is essential for genetic conservation and breeding programs; however, the current methods result in a significant loss of viability. This study investigated the potential of Eurycoma longifolia (EL) extract and its cyclodextrin complex (EL-loaded cyclodextrin [ELC]) to improve rooster sperm cryopreservation. Supplementation with EL extract at 4 mg/mL significantly increased post-thaw total motility (62.84 ± 4.45 %), progressive motility (32.70 ± 2.48 %), viability (55.90 ± 0.72 %), and decreased MDA (2.98 ± 0.41 nmol/mL) compared to controls (P < 0.05). ELC (2 mg/mL) resulted in greater improvements in total motility (59.21± 1.03 %), progressive motility (38.46± 1.43 %), and viability (58.11± 0.58 %), and lower MDA levels (2.60 ± 0.04 nmol/mL) compared to control (P < 0.05). Both EL and ELC significantly improved fertility (55.38± 1.26 % and 52.19 ± 1.09 %, respectively; P < 0.05). These results highlight the potential of EL and ELC for optimizing cryopreservation protocols.
公鸡精液的冷冻保存对于遗传保护和育种计划至关重要；然而，目前的方法会导致活力显著丧失。本研究调查了长叶刺蒺藜（EL）提取物及其环糊精复合物（负载EL的环糊精[ELC]）改善公鸡精子冷冻保存的潜力。与对照组相比，添加4 mg/mL的EL提取物显著提高了解冻后的总活力（62.84±4.45%）、前进活力（32.70±2.48%）、存活率（55.90±0.72%），并降低了丙二醛（MDA）水平（2.98±0.41 nmol/mL）（P<0.05）。与对照组相比，ELC（2 mg/mL）在总活力（59.21±1.03%）、前进活力（38.46±1.43%）和存活率（58.11±0.58%）方面有更大改善，且MDA水平更低（2.60±0.04 nmol/mL）（P<0.05）。EL和ELC均显著提高了受精率（分别为55.38±1.26%和52.19±1.09%；P<0.05）。这些结果突出了EL和ELC在优化冷冻保存方案方面的潜力。
