Kitamura Masashi, Kiba Yuka, Suzuki Ryuichiro, Tomida Natsumi, Uwaya Akemi, Isami Fumiyuki, Deng Shixin
Laboratory of Pharmacognocy, School of Pharmacy, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, 1-1, Keyakidai, Sakado, Saitama 350-0295, Japan.
Research and Development, Morinda Worldwide, Inc., Morinda Bldg., 3-2-2 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.
Medicines (Basel). 2020 Sep 7;7(9):57. doi: 10.3390/medicines7090057.
Hemp ( L.) seed contains high contents of various nutrients, including fatty acids and proteins. Cannabidiol (CBD) is a non-psychoactive compound that can be extracted from and used for treating epilepsy and pain. Industrial hemp products, including CBD and hemp seed oils, have become increasingly popular. Some products are marketed without a clear distinction between CBD and hemp seed oils. Herein, the CBD content and biological activities of commercial CBD and hemp seed oils were examined. CBD content was measured by high-performance liquid chromatography. For in vitro antioxidant activity determination, 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radical-scavenging assays were performed. The CBD concentrations in the two CBD oil samples were 18.9 ± 0.5 and 9.2 ± 0.4 mg/mL. Of the seven hemp seed oil samples, six samples contained CBD in concentrations ranging from 2.0 ± 0.1 to 20.5 ± 0.5 µg/mL, but it was not detected in one sample. Antioxidant activity was observed in both CBD oil samples. The results indicate that (1) CBD content varied by hemp seed oil sample and that (2) antioxidant activity could be a useful landmark for discriminating CBD oils from hemp seed oils.
大麻（L.）种子含有多种营养成分，包括脂肪酸和蛋白质。大麻二酚（CBD）是一种无精神活性的化合物，可从大麻中提取并用于治疗癫痫和疼痛。包括CBD和大麻籽油在内的工业大麻产品越来越受欢迎。一些产品在销售时没有明确区分CBD和大麻籽油。在此，对市售CBD和大麻籽油的CBD含量及生物活性进行了检测。通过高效液相色谱法测定CBD含量。为了测定体外抗氧化活性，进行了2,2-二苯基-1-苦基肼和2,2'-偶氮二（3-乙基苯并噻唑啉-6-磺酸）自由基清除试验。两个CBD油样品中的CBD浓度分别为18.9±0.5和9.2±0.4毫克/毫升。在七个大麻籽油样品中，六个样品的CBD浓度在2.0±0.1至20.5±0.5微克/毫升之间，但有一个样品未检测到。两个CBD油样品均观察到抗氧化活性。结果表明：（1）不同大麻籽油样品的CBD含量不同；（2）抗氧化活性可能是区分CBD油和大麻籽油的有用标志。
