线粒体钙/钠反向转运体催化的交换反应的化学计量学。
The stoichiometry of the exchange catalysed by the mitochondrial calcium/sodium antiporter.
作者信息
Brand M D
出版信息
Biochem J. 1985 Jul 1;229(1):161-6. doi: 10.1042/bj2290161.
Abstract
Rat heart mitochondria respiring on succinate in the presence of Ruthenium Red (to inhibit uptake on the Ca2+ uniporter) released Ca2+ on the calcium/sodium antiporter until a steady state was reached. Addition of the ionophore A23187 (which catalyses Ca2+/2H+ exchange) did not perturb this steady state. Thermodynamic analysis showed that if a Ca2+/nNa+ exchange with any value of n other than 2 was at equilibrium, addition of A23187 would cause an obvious change in extramitochondrial free [Ca2+]. Therefore the endogenous calcium/sodium antiporter of mitochondria catalyses electroneutral Ca2+/2Na+ exchange.
摘要
在钌红（抑制钙离子单向转运体摄取）存在的情况下，以琥珀酸为呼吸底物的大鼠心脏线粒体通过钙/钠反向转运体释放钙离子，直至达到稳态。添加离子载体A23187（催化Ca2+/2H+交换）并未扰乱该稳态。热力学分析表明，如果n值不为2的Ca2+/nNa+交换处于平衡状态，添加A23187会导致线粒体外游离[Ca2+]发生明显变化。因此，线粒体的内源性钙/钠反向转运体催化电中性的Ca2+/2Na+交换。
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