School of Public Health, Jilin Medical University, Jilin 132013, PR China; and College of Life Sciences, Jilin Agricultural University, Changchun 130118, PR China.
College of Life Sciences, Jilin Agricultural University, Changchun 130118, PR China.
Funct Plant Biol. 2024 Sep;51. doi: 10.1071/FP23161.
In recent years, alkaline soda soil has stimulated numerous biological research on plants under carbonate stress. Here, we explored the difference in physiological regulation of rice seedlings between saline (NaCl) and alkaline carbonate (NaHCO3 and Na2 CO3 ) stress. The rice seedlings were treated with 40mM NaCl, 40mM NaHCO3 and 20mM Na2 CO3 for 2h, 12h, 24h and 36h, their physiological characteristics were determined, and organic acid biosynthesis and metabolism and hormone signalling were identified by transcriptome analysis. The results showed that alkaline stress caused greater damage to their photosynthetic and antioxidant systems and led to greater accumulation of organic acid, membrane damage, proline and soluble sugar but a decreased jasmonic acid content compared with NaCl stress. Jasmonate ZIM-Domain (JAZ), the probable indole-3-acetic acid-amido synthetase GH3s, and the protein phosphatase type 2Cs that related to the hormone signalling pathway especially changed under Na2 CO3 stress. Further, the organic acid biosynthesis and metabolism process in rice seedlings were modified by both Na2 CO3 and NaHCO3 stresses through the glycolate/glyoxylate and pyruvate metabolism pathways. Collectively, this study provides valuable evidence on carbonate-responsive genes and insights into the different molecular mechanisms of saline and alkaline stresses.
近年来，堿性苏打土激发了人们对碳酸盐胁迫下植物的大量生物学研究。在这里，我们探讨了水稻幼苗在盐（NaCl）和堿性碳酸盐（NaHCO3 和 Na2 CO3 ）胁迫下的生理调节差异。用 40mM NaCl、40mM NaHCO3 和 20mM Na2 CO3 处理水稻幼苗 2h、12h、24h 和 36h，测定其生理特性，并通过转录组分析鉴定有机酸生物合成和代谢及激素信号。结果表明，堿性胁迫对其光合和抗氧化系统造成更大的损害，导致有机酸、膜损伤、脯氨酸和可溶性糖积累更大，而茉莉酸含量比 NaCl 胁迫降低。茉莉酸 ZIM 结构域（JAZ）、可能的吲哚-3-乙酸酰胺合成酶 GH3s 和与激素信号通路有关的蛋白磷酸酶 2C 在 Na2 CO3 胁迫下变化尤其明显。此外，Na2 CO3 和 NaHCO3 胁迫通过甘氨酸/乙醛酸和丙酮酸代谢途径改变了水稻幼苗的有机酸生物合成和代谢过程。总之，这项研究为碳酸响应基因提供了有价值的证据，并深入了解了盐和堿胁迫的不同分子机制。
