Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Department of Horticulture, Northeast Agricultural University, Harbin 150030, China.
Institute of Eco-Environment and Plant Protection, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
J Appl Microbiol. 2024 Sep 2;135(9). doi: 10.1093/jambio/lxae243.
This study aimed to assess the effects of phenolic acid-degrading bacteria strains on phenolic acid content, plant growth, and soil bacterial community in phenolic acid-treated soils.
The strain of interest coded as B55 was isolated from cucumber root litter, and its degradation rates of ferulic acid and p-coumaric acid were 81.92% and 72.41% in Luria-Bertani solution, respectively, and B55 was identified as Bacillus subtilis. B55 had plant growth-promoting attributes, including solubilization of inorganic phosphate and production of siderophore and indole acetic acid. Both ferulic acid and p-coumaric acid significantly restrained an increase in cucumber seedling dry biomass, while the B55 inoculation not only completely counteracted the damage of phenolic acids to cucumber seedlings and decreased the content of ferulic acid and p-coumaric acid in soil, but also promoted cucumber seedlings growth. Amplicon sequencing found that B55 inoculation changed the cucumber rhizosphere bacterial community structure and promoted the enrichment of certain bacteria, such as Pseudomonas, Arthrobacter, Bacillus, Flavobacterium, Streptomyces, and Comamonas.
B55 not only promoted cucumber seedling growth, and decreased the content of ferulic acid and p-coumaric acid in soil, but it also increased the relative abundance of beneficial microorganisms in the cucumber rhizosphere.
本研究旨在评估酚酸降解菌对酚酸处理土壤中酚酸含量、植物生长和土壤细菌群落的影响。
从黄瓜根屑中分离出一株感兴趣的菌株，编号为 B55，其在 Luria-Bertani 溶液中对阿魏酸和对香豆酸的降解率分别为 81.92%和 72.41%，B55 被鉴定为枯草芽孢杆菌。B55 具有植物促生特性，包括无机磷酸盐的溶解、铁载体和吲哚乙酸的产生。阿魏酸和对香豆酸都显著抑制了黄瓜幼苗干生物量的增加，而 B55 的接种不仅完全抵消了酚酸对黄瓜幼苗的损害，降低了土壤中阿魏酸和对香豆酸的含量，而且促进了黄瓜幼苗的生长。扩增子测序发现，B55 的接种改变了黄瓜根际细菌群落结构，促进了某些细菌的富集，如假单胞菌、节杆菌、芽孢杆菌、黄杆菌、链霉菌和丛毛单胞菌。
B55 不仅促进了黄瓜幼苗的生长，降低了土壤中阿魏酸和对香豆酸的含量，而且增加了黄瓜根际有益微生物的相对丰度。
