Department of Resources and Environment, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Curr Microbiol. 2024 Sep 16;81(11):358. doi: 10.1007/s00284-024-03876-6.
Antagonistic bacterial strains from Bacillus spp. have been widely studied and utilized in the biocontrol of phytopathogens and the promotion of plant growth, but their impacts on the rhizosphere microecology when applied to crop plants are unclear. Herein, the effects of applying the antagonistic bacterium Bacillus subtilis S1 as a biofertilizer on the rhizosphere microecology of cucumbers were investigated. In a pot experiment on cucumber seedlings inoculated with S1, 3124 bacterial operational taxonomic units (OTUs) were obtained from the rhizosphere soils using high-throughput sequencing of 16S rRNA gene amplicons, and the most abundant phylum was Proteobacteria that accounted for 49.48% in the bacterial community. S1 treatment significantly reduced the abundances of soil bacterial taxa during a period of approximately 30 days but did not affect bacterial diversity in the rhizosphere soils of cucumbers. The enzymatic activities of soil nitrite reductase (S-Nir) and dehydrogenase (S-DHA) were significantly increased after S1 fertilization. However, the activities of soil urease (S-UE), cellulase (S-CL), and sucrase (S-SC) were significantly reduced compared to the control group. Additionally, the ammonium- and nitrate-nitrogen contents of S1-treated soil samples were significantly lower than those of the control group. S1 fertilization reshaped the rhizosphere soil bacterial community of cucumber plants. The S-CL activity and nitrate-nitrogen content in rhizosphere soil affected by S1 inoculation play important roles in altering the abundance of rhizosphere soil microbiota.
从芽孢杆菌属中分离得到的拮抗细菌已被广泛研究和应用于植物病原菌的生物防治和植物生长的促进，但它们在应用于作物植物时对根际微生态的影响尚不清楚。本研究中，以拮抗菌枯草芽孢杆菌 S1 作为生物肥料，研究了其对黄瓜根际微生态的影响。在黄瓜幼苗接种 S1 的盆栽试验中，通过高通量测序 16S rRNA 基因扩增子，从根际土壤中获得了 3124 个细菌操作分类单元（OTUs），最丰富的门是占细菌群落 49.48%的变形菌门。S1 处理在大约 30 天的时间内显著降低了土壤细菌分类群的丰度，但对黄瓜根际土壤的细菌多样性没有影响。S1 施肥后土壤亚硝酸还原酶（S-Nir）和脱氢酶（S-DHA）的酶活性显著增加。然而，与对照组相比，土壤脲酶（S-UE）、纤维素酶（S-CL）和蔗糖酶（S-SC）的活性显著降低。此外，S1 处理土壤样品中的铵态氮和硝态氮含量明显低于对照组。S1 施肥重塑了黄瓜植物的根际土壤细菌群落。受 S1 接种影响的根际土壤 S-CL 活性和硝态氮含量在改变根际土壤微生物群落丰度方面发挥着重要作用。
