Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China.
National Technology Innovation Center of Synthetic Biology, Tianjin, 300308, People's Republic of China.
Sci Rep. 2021 Jun 8;11(1):12055. doi: 10.1038/s41598-021-91399-6.
Plant growth-promoting bacteria (PGPB) inoculants have been applied worldwide. However, the ecological roles of PGPB under different soil conditions are still not well understood. The present study aimed to explore the ecological roles of Bacillus amyloliquefaciens FH-1 (FH) on cucumber seedlings, rhizosphere soil properties, and the bacterial community in pot experiments. The results showed that FH had significant effects on cucumber seedlings and the rhizosphere bacterial community but not on soil properties. The FH promoted cucumber seedlings growth, reduced the rhizosphere bacterial diversity, increased Proteobacteria, and decreased Acidobacteria. Linear discriminant analysis (LDA) effect size (LEfSe) revealed that FH enriched two taxa (GKS2_174 and Nannocystaceae) and inhibited 18 taxa (mainly Acidobacteria, Actinobacteria, BRC1, Chloroflexi, Plantctomycetes, and Verrucomicrobia). Co-occurrence network analysis demonstrated that FH increased bacteria-bacteria interactions and that Bacillus (genus of FH) had few interactions with the enriched and inhibited taxa. This might indicate that FH does not directly affect the enriched and inhibited taxa. Correlation analysis results displayed that cucumber seedlings' weight and height/length (except root length) were significantly correlated with the 18 inhibited taxa and the enriched taxa Nannocystaceae. It was speculated that FH might promote cucumber seedling growth by indirectly enriching Nannocystaceae and inhibiting some taxa from Acidobacteria, Actinobacteria, BRC1, Chloroflexi, Plantctomycetes, and Verrucomicrobia.
植物促生菌（PGPB）接种剂已在全球范围内应用。然而，不同土壤条件下 PGPB 的生态作用仍不清楚。本研究旨在通过盆栽试验探索解淀粉芽孢杆菌 FH-1（FH）对黄瓜幼苗、根际土壤特性和细菌群落的生态作用。结果表明，FH 对黄瓜幼苗和根际细菌群落有显著影响，但对土壤性质没有影响。FH 促进了黄瓜幼苗的生长，降低了根际细菌多样性，增加了变形菌门，减少了酸杆菌门。线性判别分析（LDA）效应大小（LEfSe）显示，FH 富集了两个分类群（GKS2_174 和 Nannocystaceae），抑制了 18 个分类群（主要是酸杆菌门、放线菌门、BRC1、绿弯菌门、浮霉菌门和疣微菌门）。共现网络分析表明，FH 增加了细菌间的相互作用，而 FH 中的芽孢杆菌属与富集和抑制的分类群几乎没有相互作用。这可能表明 FH 不会直接影响富集和抑制的分类群。相关性分析结果显示，黄瓜幼苗的重量和高度/长度（除根长外）与 18 个抑制分类群和富营养分类群 Nannocystaceae 显著相关。推测 FH 可能通过间接富集 Nannocystaceae 和抑制某些来自酸杆菌门、放线菌门、BRC1、绿弯菌门、浮霉菌门和疣微菌门的分类群来促进黄瓜幼苗的生长。
