Department of Plastic Surgery and Burn Center, Second Affiliated Hospital, Shantou University Medical College, DongXiaBei Road, Shantou, 515000, Guangdong, China.
Plastic Surgery Institute of Shantou University Medical College, DongXiaBei Road, Shantou, 515000, Guangdong, China.
Sci Rep. 2024 Sep 19;14(1):21868. doi: 10.1038/s41598-024-73036-0.
Cleft palate (CP) is a congenital condition characterized by a complex etiology and limited diagnostic and therapeutic options. In this study, we delved into the molecular mechanisms associated with retinoic acid (RA)-induced CP in Kun Ming mice. Proteomic analysis of control and RA-induced CP samples at embryonic day 15.5 revealed 25 upregulated and 19 downregulated proteins. Further analysis identified these differentially expressed proteins (DEPs) as being involved in extracellular matrix organization, actin cytoskeleton, and myosin complex. Moreover, these DEPs were found to be enriched in pathways related to motor protein activity and extracellular matrix-receptor interaction. Protein-protein interaction network analysis identified 10 hub proteins, including motor proteins and ECM-related proteins, which exhibited higher expression levels in CP compared to control tissues. These findings provide insights into the molecular mechanisms underlying CP and highlight potential targets for diagnostic and therapeutic purposes.
腭裂是一种先天性疾病，具有复杂的病因和有限的诊断及治疗选择。在这项研究中，我们深入研究了维甲酸（RA）诱导昆明小鼠腭裂的分子机制。对胚胎第 15.5 天的对照和 RA 诱导腭裂样本进行蛋白质组学分析，发现 25 个上调蛋白和 19 个下调蛋白。进一步分析表明，这些差异表达蛋白（DEPs）参与细胞外基质组织、肌动蛋白细胞骨架和肌球蛋白复合物。此外，这些 DEPs 在与运动蛋白活性和细胞外基质-受体相互作用相关的途径中富集。蛋白质-蛋白质相互作用网络分析鉴定出 10 个枢纽蛋白，包括运动蛋白和 ECM 相关蛋白，它们在 CP 组织中的表达水平高于对照组织。这些发现为 CP 的分子机制提供了新的见解，并为诊断和治疗目的提供了潜在的靶点。
