Gidney M A, Bundle D R, Godard A, Griffiths B W
Can J Biochem Cell Biol. 1985 Jul;63(7):737-42. doi: 10.1139/o85-092.
Six monoclonal antibodies were developed to pregnancy-specific beta 1-glycoprotein (PS beta 1G). Studies of ascitic fluid antibodies by a double-antibody radioimmunoassay (RIA) included an evaluation of titers, dose-response parameters, and mass action properties. Four of the antibodies demonstrated moderate to high titers ranging from 1/40 000 to greater than 1/120 000, as determined by the specific binding of 125I-labeled PS beta 1G. In inhibition studies utilizing a standard containing known quantities of placental PS beta 1G, two of the antibodies (AR#11 and B#2) were highly sensitive and only slightly lower in this regard than a high affinity polyclonal antiserum. The binding affinities of AR#11 and B#2 monoclonals were greater than 10(9) mol-1 which underline their importance as potential clinical reagents for the RIA of PS beta 1G. The Scatchard plots, for several of the antibodies, were linear and in full agreement with a single order of binding sites predicted for specific monoclonal reagents. Immunodiffusion results provide preliminary evidence that at least three distinct determinants on PS beta 1G are recognized by a number of the monoclonal antibodies. Further studies on the fine specificities of the antibodies by solid-phase RIA, as well as a detailed evaluation of their clinical applications, are in progress.
已开发出六种针对妊娠特异性β1-糖蛋白（PSβ1G）的单克隆抗体。通过双抗体放射免疫测定法（RIA）对腹水抗体进行的研究包括对滴度、剂量反应参数和质量作用特性的评估。通过125I标记的PSβ1G的特异性结合测定，其中四种抗体表现出中等至高滴度，范围从1/40000至大于1/120000。在利用含有已知量胎盘PSβ1G的标准品进行的抑制研究中，两种抗体（AR#11和B#2）高度敏感，在这方面仅略低于高亲和力多克隆抗血清。AR#11和B#2单克隆抗体的结合亲和力大于10(9)mol-1，这突出了它们作为PSβ1G放射免疫测定潜在临床试剂的重要性。几种抗体的Scatchard图呈线性，与特定单克隆试剂预测的单一结合位点顺序完全一致。免疫扩散结果提供了初步证据，表明PSβ1G上至少有三个不同的决定簇被多种单克隆抗体识别。正在通过固相RIA对抗体的精细特异性进行进一步研究，以及对其临床应用进行详细评估。
