用单克隆抗体免疫吸附剂纯化妊娠特异性β1-糖蛋白

Purification of pregnancy-specific beta 1-glycoprotein with a monoclonal antibody immunoadsorbent.

作者信息

Heikinheimo M, Stenman U H, Bång B, Hurme M, Mäkelä O, Bohn H

出版信息

J Immunol Methods. 1983 May 27;60(1-2):25-31. doi: 10.1016/0022-1759(83)90331-9.

Abstract

Hybrid cell lines secreting monoclonal antibodies against pregnancy-specific beta 1-glycoprotein (SP1) were produced by fusion of a mouse myeloma cell line with spleen cells from BALB/c mice immunized with purified placental SP1. The clones were further grown intraperitoneally in mice, and the ascites fluids contained anti-SP1 antibodies in high titers. One of the monoclonal antibodies was coupled to cyanogen-bromide-activated Sepharose and used for affinity chromatography of SP1 using late pregnancy serum as starting material. The purification of SP1 by means of immunoadsorption utilizing monoclonal antibodies offers remarkable advantages compared with the purification procedures used so far.

摘要

通过将小鼠骨髓瘤细胞系与用纯化的胎盘妊娠特异性β1-糖蛋白（SP1）免疫的BALB/c小鼠的脾细胞融合，产生了分泌抗妊娠特异性β1-糖蛋白单克隆抗体的杂交细胞系。这些克隆在小鼠体内进一步进行腹腔培养，腹水含有高滴度的抗SP1抗体。其中一种单克隆抗体与溴化氰活化的琼脂糖偶联，并用于以妊娠晚期血清为起始原料的SP1亲和层析。与目前使用的纯化方法相比，利用单克隆抗体通过免疫吸附纯化SP1具有显著优势。