College of Agriculture, Shanxi Agricultural University, Key Laboratory of Sustainable Dryland Agriculture (Co-construction by Ministry and Province), Jinzhong, China.
College of Life Sciences, Shanxi Agricultural University, Jinzhong, China.
PeerJ. 2024 Sep 16;12:e17778. doi: 10.7717/peerj.17778. eCollection 2024.
Seed vigor recognized as a quantitative trait is of particular importance for agricultural production. However, limited knowledge is available for understanding genetic basis of wheat seed vigor.
The aim of this study was to identify quantitative trait loci (QTL) responsible for 10 seed vigor-related traits representing multiple aspects of seed-vigor dynamics during artificial aging with 6 different treatment times (0, 24, 36, 48, 60, and 72 h) under controlled conditions (48 °C, 95% humidity, and dark). The mapping populations were two wheat introgression lines (IL-1 and IL-2) derived from recipient parent (Lumai 14) and donor parent (Shaanhan 8675 or Jing 411).
A total of 26 additive QTLs and 72 pairs of epistatic QTLs were detected for wheat seed-vigor traits. Importantly, chromosomes 1B and 7B contained several co-located QTLs, and chromosome 2A had a QTL-rich region near the marker Xwmc667, indicating that these QTLs may affect wheat seed vigor with pleiotropic effects. Furthermore, several possible consistent QTLs (hot-spot regions) were examined by comparison analysis of QTLs detected in this study and reported previously. Finally, a set of candidate genes for wheat seed vigor were predicted to be involved in transcription regulation, carbohydrate and lipid metabolism.
The present findings lay new insights into the mechanism underlying wheat seed vigor, providing valuable information for wheat genetic improvement especially marker-assisted breeding to increase seed vigor and consequently achieve high grain yield despite of further investigation required.
种子活力作为一种数量性状，对农业生产具有重要意义。然而，人们对小麦种子活力的遗传基础知之甚少。
本研究旨在鉴定与种子活力相关的 10 个数量性状的数量性状位点（QTL），这些性状代表了在受控条件下（48°C、95%湿度和黑暗）人工老化过程中种子活力动态的多个方面，处理时间为 6 个不同时间（0、24、36、48、60 和 72 h）。作图群体由来自受体亲本（Lumai 14）和供体亲本（Shaanhan 8675 或 Jing 411）的两个小麦导入系（IL-1 和 IL-2）组成。
共检测到 26 个加性 QTL 和 72 对上位性 QTL 与小麦种子活力性状相关。重要的是，染色体 1B 和 7B 包含几个共定位的 QTL，染色体 2A 在标记 Xwmc667 附近有一个富含 QTL 的区域，表明这些 QTL 可能通过多效性影响小麦种子活力。此外，通过比较本研究和以前报道中检测到的 QTL 的分析，检查了几个可能的一致 QTL（热点区域）。最后，预测了一组与小麦种子活力相关的候选基因，这些基因可能参与转录调控、碳水化合物和脂质代谢。
本研究结果为小麦种子活力的机制提供了新的见解，为小麦遗传改良特别是标记辅助选择提供了有价值的信息，以增加种子活力，从而在进一步研究的情况下实现高产。
